論文 - 詳細
| RRC ID | 43765 |
|---|---|
| 著者 | Kitano T, Yoda H, Tabata K, Miura M, Toriyama M, Motohashi S, Suzuki T. |
| タイトル | Vitamin K3 analogs induce selective tumor cytotoxicity in neuroblastoma. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
We investigated the cytotoxicity of eight vitamin K3 (VK3) analogs against neuroblastoma cell lines (IMR-32, LA-N-1, NB-39, and SK-N-SH) and normal cell lines (human umbilical vein endothelial cells (HUVEC) and human dermal fibroblasts (HDF)) using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. 2-[(2-Methoxy)ethylthio]-3-methyl-1,4-naphthoquinone (VK3-OCH(3)) showed especially potent cytotoxic activities against neuroblastoma cells compared with normal cells. In a Hoechst 33342 staining experiment, apoptotic morphologies characterized by cell shrinkage, nuclear condensation, and nuclear fragmentation were observed in IMR-32 and LA-N-1 cells after 48 h of treatment with 10(-5) M of VK3-OCH(3). To clarify the molecular mechanisms of apoptosis induced by VK3-OCH(3), we examined the expression of apoptosis related proteins using a Proteome Profiler Array and western blotting. Heme oxygenase (HO)-1 was remarkably increased by VK3-OCH(3) compared with the control (173% in IMR-32 and 170% in LA-N-1 at 24 h). Moreover, caveolin-1 was induced by VK3-OCH(3) at 48 h. In addition, VK3-OCH(3) arrested the cell cycle at the G2/M phase in IMR-32 cells. These results suggest that VK3-OCH(3) exhibited a selective antitumor activity via HO-1-related mechanisms. |
| 巻・号 | 35(4) |
| ページ | 617-23 |
| 公開日 | 2012-1-1 |
| DOI | 10.1248/bpb.35.617 |
| PII | JST.JSTAGE/bpb/35.617 |
| PMID | 22466570 |
| MeSH | Antineoplastic Agents / pharmacology* Caveolin 1 Cell Cycle / drug effects Cell Line, Tumor Cell Survival Fibroblasts / drug effects Fibroblasts / metabolism Fibroblasts / pathology Gene Expression Profiling Heme Oxygenase-1 Human Umbilical Vein Endothelial Cells Humans Neuroblastoma / drug therapy* Neuroblastoma / metabolism Neuroblastoma / pathology Proteome Vitamin K 3 / analogs & derivatives* Vitamin K 3 / pharmacology |
| IF | 1.863 |
| 引用数 | 17 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LA-N-1(RCB0483) SK-N-SH(RCB0426) |