| RRC ID |
43768
|
| 著者 |
Kang W, Tong JH, Chan AW, Lung RW, Chau SL, Wong QW, Wong N, Yu J, Cheng AS, To KF.
|
| タイトル |
Stathmin1 plays oncogenic role and is a target of microRNA-223 in gastric cancer.
|
| ジャーナル |
PLoS One
|
| Abstract |
Stathmin1 (STMN1) is a candidate oncoprotein and prognosis marker in several kinds of cancers. This study was aimed to analyze its expression and biological functions in gastric cancer. The expression of STMN1 was evaluated by qRT-PCR, western blot and immunohistochemistry. The biological function of STMN1 was determined by MTT proliferation assays, monolayer colony formation and cell invasion assays using small interference RNA technique in gastric cancer cell lines. We also explored the regulation of STMN1 expression by microRNA-223. STMN1 was upregulated in gastric cancer cell lines and primary gastric adenocarcinomas. STMN1-positive tumors were more likely to be found in old age group and associated with p53 nuclear expression. In diffuse type gastric adenocarcinomas, STMN1 expression was correlated with age (p = 0.043), T stage (p = 0.004) and lymph node metastasis (p = 0.046). Expression of STMN1 in diffuse type gastric adenocarcinoma was associated with poor disease specific survival by univariate analysis (p = 0.01). STMN1 knockdown in AGS and MKN7 cell lines suppressed proliferation (p<0.001), reduced monolayer colony formation (p<0.001), inhibited cell invasion and migration ability (p<0.001) and induced G1 phase arrest. siSTMN1 could also suppress cell growth in vivo (p<0. 01). We finally confirmed that STMN1 is a putative downstream target of miR-223 in gastric cancer. Our findings supported an oncogenic role of STMN1 in gastric cancer. STMN1 might serve as a prognostic marker and a potential therapeutic target for gastric cancer.
|
| 巻・号 |
7(3)
|
| ページ |
e33919
|
| 公開日 |
2012-1-1
|
| DOI |
10.1371/journal.pone.0033919
|
| PII |
PONE-D-11-20480
|
| PMID |
22470493
|
| PMC |
PMC3314670
|
| MeSH |
Adenocarcinoma / metabolism*
Adenocarcinoma / mortality
Adenocarcinoma / pathology
Aged
Cell Line, Tumor
Cell Movement
Female
G1 Phase Cell Cycle Checkpoints
Humans
Male
MicroRNAs / metabolism*
Middle Aged
Prognosis
RNA Interference
RNA, Small Interfering / metabolism
Stathmin / antagonists & inhibitors
Stathmin / genetics
Stathmin / metabolism*
Stomach Neoplasms / metabolism*
Stomach Neoplasms / mortality
Stomach Neoplasms / pathology
|
| IF |
2.74
|
| 引用数 |
66
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MKN28(RCB1000)
Kato III(RCB2088)
MKN45(RCB1001)
MKN7(RCB0999)
MKN1(RCB1003) |
