RRC ID 43836
著者 Sahmi F, Nicola E, Price CA.
タイトル Bioassay for follicle stimulating activity of equine gonadotropic hormone in mare serum using frozen/thawed transiently transfected reporter cells.
ジャーナル Theriogenology
Abstract The objective was to establish a cell line-based bioassay for FSH in horse serum for screening samples with high eCG bioactivity. A cell line (HEK293) was transiently cotransfected with an FSH reporter expression plasmid and a cAMP-responsive β-galactosidase reporter plasmid. Cells were bulk frozen, and thawed for assay purposes. This assay was specific for FSH, with no cross-reaction with LH or insulin-like growth factor-1. Standard curves (eCG) and serum samples from pregnant mares passed parallel line bioassay validity tests (linearity and parallelism). Estimates of bioactivity with this bioassay were highly correlated with estimates obtained with the Steelman-Pohley hCG augmentation assay. The colorimetric end point permitted the use of this assay as a rapid screen for FSH bioactivity without the need for animal use or complex cell culture facilities.
巻・号 78(4)
ページ 724-30
公開日 2012-9-1
DOI 10.1016/j.theriogenology.2012.03.015
PII S0093-691X(12)00186-0
PMID 22578627
MeSH Animals Biological Assay / methods* Biological Assay / veterinary Cells, Cultured Dose-Response Relationship, Drug Female Follicle Stimulating Hormone / analysis* Follicle Stimulating Hormone / blood* Follicle Stimulating Hormone / metabolism Follicle Stimulating Hormone / pharmacology Freezing Genes, Reporter / physiology Gonadotropins, Equine / analysis* Gonadotropins, Equine / blood Gonadotropins, Equine / metabolism Gonadotropins, Equine / pharmacology HEK293 Cells Horses / blood* Horses / metabolism Humans Transfection
IF 2.094
引用数 0
WOS 分野 REPRODUCTIVE BIOLOGY VETERINARY SCIENCES
リソース情報
ヒト・動物細胞 KGN(RCB1154)