論文 - 詳細
| RRC ID | 43845 |
|---|---|
| 著者 | Itoh T, Ando M, Tsukamasa Y, Akao Y. |
| タイトル | Expression of BMP-2 and Ets1 in BMP-2-stimulated mouse pre-osteoblast differentiation is regulated by microRNA-370. |
| ジャーナル | FEBS Lett |
| Abstract |
MicroRNAs (miRs) regulate several biological functions such as cell growth, cell differentiation, and carcinogenesis, by binding to the 3'-untranslated regions (3'-UTR) of specific target genes, in order to repress translation or promote degradation of the transcribed mRNAs. In the present study, using microRNA array and in silico analyses, we found that miR-370 regulates the expression of bone morphogenetic protein-2 (BMP-2) and V-ets Erythroblastosis Virus E26 Oncogene Homolog 1 (Ets1) in BMP-2-stimulated murine pre-osteoblast MC3T3-E1 cell differentiation. The enforced expression of mature miR-370 in MC3T3-E1 cells or primary osteoblast cells remarkably attenuated BMP-2-induced pre-osteoblast differentiation. To ascertain the mechanisms underlying the regulation of osteoblast differentiation by miR-370, we hypothesized a BMP-2-Ets1-PTHrP feed-forward loop regulatory mechanism. |
| 巻・号 | 586(12) |
| ページ | 1693-701 |
| 公開日 | 2012-6-12 |
| DOI | 10.1016/j.febslet.2012.04.014 |
| PII | S0014-5793(12)00292-X |
| PMID | 22587915 |
| MeSH | Animals Base Pairing Base Sequence Bone Morphogenetic Protein 2 / genetics Bone Morphogenetic Protein 2 / metabolism* Bone Morphogenetic Protein 2 / physiology Cell Differentiation / genetics* Cell Line Core Binding Factor Alpha 1 Subunit / genetics Core Binding Factor Alpha 1 Subunit / metabolism Down-Regulation Gene Expression Profiling Genes, Reporter Luciferases, Renilla / biosynthesis Luciferases, Renilla / genetics Mice MicroRNAs / genetics MicroRNAs / metabolism MicroRNAs / physiology* Osteoblasts / physiology* Osteopontin / genetics Osteopontin / metabolism Protein Biosynthesis Proto-Oncogene Protein c-ets-1 / genetics Proto-Oncogene Protein c-ets-1 / metabolism* RNA Interference* Transcription, Genetic |
| IF | 3.057 |
| 引用数 | 26 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |