論文 - 詳細
| RRC ID | 43892 |
|---|---|
| 著者 | Takahashi M, Elbarbary RA, Abe M, Sato M, Yoshida T, Yamada Y, Tamura M, Nashimoto M. |
| タイトル | Elimination of specific miRNAs by naked 14-nt sgRNAs. |
| ジャーナル | PLoS One |
| Abstract |
tRNase Z(L)-utilizing efficacious gene silencing (TRUE gene silencing) is a newly developed technology to suppress mammalian gene expression. TRUE gene silencing works on the basis of a unique enzymatic property of mammalian tRNase Z(L), which is that it can recognize a pre-tRNA-like or micro-pre-tRNA-like complex formed between target RNA and artificial small guide RNA (sgRNA) and can cleave any target RNA at any desired site. There are four types of sgRNA, 5'-half-tRNA, RNA heptamer, hook RNA, and ~14-nt linear RNA. Here we show that a 14-nt linear-type sgRNA against human miR-16 can guide tRNase Z(L) cleavage of miR-16 in vitro and can downregulate the miR-16 level in HEK293 cells. We also demonstrate that the 14-nt sgRNA can be efficiently taken up without any transfection reagents by living cells and can exist stably in there for at least 24 hours. The naked 14-nt sgRNA significantly reduced the miR-16 level in HEK293 and HL60 cells. Three other naked 14-nt sgRNAs against miR-142-3p, miR-206, and miR-19a/b are also shown to downregulate the respective miRNA levels in various mammalian cell lines. Our observations suggest that in general we can eliminate a specific cellular miRNA at least by ~50% by using a naked 14-nt sgRNA on the basis of TRUE gene silencing. |
| 巻・号 | 7(6) |
| ページ | e38496 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0038496 |
| PII | PONE-D-11-24813 |
| PMID | 22675569 |
| PMC | PMC3366928 |
| MeSH | Base Sequence Cell Line Cell Survival Down-Regulation / genetics Endoribonucleases Gene Silencing Humans MicroRNAs / genetics MicroRNAs / metabolism* Molecular Sequence Data Nucleotides / metabolism* RNA, Guide, Kinetoplastida / genetics RNA, Guide, Kinetoplastida / metabolism* |
| IF | 2.74 |
| 引用数 | 8 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293(RCB1637) HeLa(RCB0007) Jurkat(RCB0806) HL60(RCB0041) DAUDI |