| RRC ID |
43940
|
| 著者 |
Watanabe T, Tamamura Y, Hoshino A, Makino Y, Kamioka H, Amagasa T, Yamaguchi A, Iimura T.
|
| タイトル |
Increasing participation of sclerostin in postnatal bone development, revealed by three-dimensional immunofluorescence morphometry.
|
| ジャーナル |
Bone
|
| Abstract |
Confocal immunofluorescence tiling imaging revealed the spatio-temporal distributions of osterix and sclerostin in femurs from 3-day-old, 2-week-old and 4-week-old rats to be reciprocally exclusive at the tissue level. Further quantitative three-dimensional immuno fluorescence morphometry demonstrated the increasing distribution of sclerostin in the osteocytic lacuno-canalicular system specifically in diaphysis, which paralleled the cooperative participation and depletion of osterix and β-catenin in adjacent periosteum cells. Treating MC3T3-E1 cells with BIO (a GSK3 inhibitor) induced the stabilization of β-catenin and nuclear translocation of osterix, and negatively regulated osteocalcin/BGLAP and Dmp1. These results collectively demonstrate that the increasing distribution of sclerostin in diaphyseal cortical bone appears to be involved in the attenuation of osterix and β-catenin in adjacent periosteum cells, thus possibly contributing to osteoblast maturation and reducing the osteoblast formation at this bone site. Our confocal microscopy-based imaging analyses provide a comprehensive and detailed view of the spatio-temporal distribution of sclerostin, β-catenin and osterix at the tissue to subcellular level in a coherent manner, and uncovered their spatio-temporal cooperation in postnatal bone development, thus providing evidence that they link skeletogenic growth and functional bone development.
|
| 巻・号 |
51(3)
|
| ページ |
447-58
|
| 公開日 |
2012-9-1
|
| DOI |
10.1016/j.bone.2012.06.019
|
| PII |
S8756-3282(12)00946-5
|
| PMID |
22766096
|
| MeSH |
Aging / metabolism
Animals
Animals, Newborn
Bone Development* / drug effects
Bone Morphogenetic Proteins / metabolism*
Cell Count
Cell Line
Cell Nucleus / drug effects
Cell Nucleus / metabolism
Core Binding Factor Alpha 1 Subunit / metabolism
Diaphyses / cytology
Diaphyses / drug effects
Diaphyses / growth & development
Diaphyses / metabolism
Femur / cytology
Femur / drug effects
Femur / growth & development
Femur / metabolism
Fluorescent Antibody Technique / methods*
Genetic Markers
Imaging, Three-Dimensional / methods*
Indoles / pharmacology
Male
Mice
Microscopy, Confocal
Osteocytes / cytology
Osteocytes / drug effects
Osteocytes / metabolism
Oximes / pharmacology
Protein Stability / drug effects
Protein Transport / drug effects
Rats
Sp7 Transcription Factor
Time Factors
Transcription Factors / metabolism
Wnt Signaling Pathway / drug effects
beta Catenin / metabolism
|
| IF |
4.147
|
| 引用数 |
8
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
