Reference - Detail
| RRC ID | 43954 |
|---|---|
| Author | Nagao H, Setoguchi T, Kitamoto S, Ishidou Y, Nagano S, Yokouchi M, Abematsu M, Kawabata N, Maeda S, Yonezawa S, Komiya S. |
| Title | RBPJ is a novel target for rhabdomyosarcoma therapy. |
| Journal | PLoS One |
| Abstract |
The Notch pathway regulates a broad spectrum of cell fate decisions and differentiation processes during fetal and postnatal development. In addition, the Notch pathway plays an important role in controlling tumorigenesis. However, the role of RBPJ, a transcription factor in the Notch pathway, in the development of tumors is largely unknown. In this study, we focused on the role of RBPJ in the pathogenesis of rhabdomyosarcoma (RMS). Our data showed that Notch pathway genes were upregulated and activated in human RMS cell lines and patient samples. Inhibition of the Notch pathway by a γ-secretase inhibitor (GSI) decreased the in vitro proliferation of RMS cells. Knockdown of RBPJ expression by RNAi inhibited the anchorage-independent growth of RMS cells and the growth of xenografts in vivo. Additionally, overexpression of RBPJ promoted the anchorage-independent growth of RMS cells. Further, we revealed that RBPJ regulated the cell cycle in RMS xenograft tumors and decreased proliferation. Our findings suggest that RBPJ regulates the RMS growth, and that the inhibition of RBPJ may be an effective therapeutic approach for patients with RMS. |
| Volume | 7(7) |
| Pages | e39268 |
| Published | 2012-1-1 |
| DOI | 10.1371/journal.pone.0039268 |
| PII | PONE-D-12-06292 |
| PMID | 22792167 |
| PMC | PMC3392254 |
| MeSH | Animals Carbamates / pharmacology Cell Line, Tumor Cell Proliferation Dibenzazepines / pharmacology Dipeptides / pharmacology Gene Expression Regulation, Neoplastic / drug effects Gene Knockdown Techniques Humans Immunoglobulin J Recombination Signal Sequence-Binding Protein / genetics* Immunoglobulin J Recombination Signal Sequence-Binding Protein / metabolism Mice Mice, Nude RNA Interference Receptors, Notch / metabolism Rhabdomyosarcoma / genetics* Rhabdomyosarcoma / pathology Rhabdomyosarcoma / therapy Signal Transduction / drug effects Tumor Burden / genetics Xenograft Model Antitumor Assays |
| IF | 2.74 |
| Times Cited | 12 |
| WOS Category | ONCOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RMS-YM(RCB1695) |