| RRC ID |
43975
|
| 著者 |
Uzawa K, Baba T, Uchida F, Yamatoji M, Kasamatsu A, Sakamoto Y, Ogawara K, Shiiba M, Bukawa H, Tanzawa H.
|
| タイトル |
Circulating tumor-derived mutant mitochondrial DNA: a predictive biomarker of clinical prognosis in human squamous cell carcinoma.
|
| ジャーナル |
Oncotarget
|
| Abstract |
While circulating tumor-derived molecules have been identified in a variety of malignant tumors, it is sometimes difficult to detect the molecular targets due to the lower serum concentration. We report that evaluation of circulating tumor-derived mitochondrial DNA (mtDNA) seems to have novel efficiency for detecting tumoral micrometastasis. In murine xenografting human oral cancer cells, human mtDNAs could be quantitatively detected from multiple organs and blood samples whereas human nucleic DNAs could not. We also determined if this mtDNA blood test was relevant for patients with oral cancer with no histologic evidence of tumoral cells in their surgical margins. For this, mtDNA from normal and tumorous tissues and serum mtDNA obtained pre and postoperatively was examined at three different regions including the displacement loop, 12S-rRNA, and 16S-rRNA. All non-recurring patients had significantly higher amounts of mutant mtDNAs in the tumoral tissues compared with the non-recurring group. More importantly, on the blood test with the cut-off point by receiver operating characteristic (ROC) curve analysis, while the vast majority of serum mtDNA samples obtained postoperatively in the recurring cases maintained significantly higher amounts of mutant mtDNAs, the non-recurring cases did not, and they showed good prognosis. This is the first report of this approach for managing patients after resection of oral tumors, and may have substantial diagnostic potential for other tumoral types.
|
| 巻・号 |
3(7)
|
| ページ |
670-7
|
| 公開日 |
2012-7-1
|
| DOI |
10.18632/oncotarget.523
|
| PII |
523
|
| PMID |
22832574
|
| PMC |
PMC3443250
|
| MeSH |
Animals
Biomarkers, Tumor / blood
Biomarkers, Tumor / genetics*
Carcinoma, Squamous Cell / blood
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / pathology
Cell Line, Tumor
DNA, Mitochondrial / blood*
DNA, Mitochondrial / genetics*
Humans
Mice
Mice, Inbred BALB C
Mouth Neoplasms / blood
Mouth Neoplasms / genetics*
Mouth Neoplasms / pathology
Mutation*
Prognosis
|
| IF |
5.168
|
| 引用数 |
18
|
|
WOS 分野
|
ONCOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Sa3(RCB0980) |
