Reference - Detail
| RRC ID | 43976 |
|---|---|
| Author | Yang CR, Chen JD. |
| Title | Preparation and biological evaluation of chitosan-collagen-icariin composite scaffolds for neuronal regeneration. |
| Journal | Neurol Sci |
| Abstract |
In this study the chitosan/collagen/icariin composite scaffolds for nerve regeneration were produced by blending and crosslinking chitosan with collagen and icariin. The microstructure of the composite scaffolds was observed by scanning electron microscopy. 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide and attachment assays were conducted, respectively, to investigate the biocompatibility of the scaffolds. Cell cultural tests showed that the channel-structured porous scaffolds acted as a positive factor to support connective nerve cell growth. After culture, cells showed a clear flow trend to move close to the composite scaffolds in culture solution, arranging in spiral-like, and aligned parallel to the orientation of the channel structure on the surface of scaffolds. When compared to pure chitosan and chitosan/collagen scaffolds, Schwann cells and PC12 cells on the chitosan/collagen/icariin composite scaffolds exhibited the greatest proliferation and longest average neurite length. These results suggested that the chitosan/collagen/icariin composite scaffolds are potential cell carriers in nerve tissue engineering. |
| Volume | 34(6) |
| Pages | 941-7 |
| Published | 2013-6-1 |
| DOI | 10.1007/s10072-012-1165-z |
| PMID | 22833221 |
| MeSH | Animals Cell Differentiation / drug effects Cell Enlargement / drug effects* Cell Proliferation / drug effects Cells, Cultured Chitosan / chemistry Chitosan / pharmacology* Collagen / chemistry Collagen / pharmacology* Flavonoids / chemistry Flavonoids / pharmacology* Mice Mice, Inbred BALB C Microscopy, Electron, Scanning Neurites / drug effects Neurites / ultrastructure PC12 Cells Rats Schwann Cells / drug effects Schwann Cells / physiology Schwann Cells / ultrastructure Sciatic Nerve / cytology Time Factors Tissue Scaffolds* |
| IF | 2.415 |
| Times Cited | 14 |
| WOS Category | CLINICAL NEUROLOGY NEUROSCIENCES |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | PC-12(RCB0009) |