RRC ID 43986
著者 Jin G, Mizutani A, Fukuda T, Chen L, Nakanishi K, Yan T, Kudoh T, Hirohata S, Kasai T, Murakami H, Salomon DS, Seno M.
タイトル Eosinophil cationic protein enhances cardiomyocyte differentiation of P19CL6 embryonal carcinoma cells by stimulating the FGF receptor signaling pathway.
ジャーナル Growth Factors
Abstract We investigated the functional role of eosinophil cationic protein (ECP) in regulating cardiomyogenesis using mouse P19CL6 embryonic carcinoma cells. ECP was confirmed to accelerate the cardiomyocyte differentiation of P19CL6 cells by enhancing the rate and area size of beating of cardiomyocyte and by facilitating the expression of cardiomyocyte-specific genes, such as GATA4 and α-MHC. Since cardiomyocyte differentiation in vivo is considered to follow mesoderm induction, the induction of Brachyury, a marker of mesoderm, was assessed. Brachyury expression was found to be enhanced after the addition of ECP. This enhancement was due to the stimulation of extracellular signal-regulated kinase (ERK)1/2 phosphorylation by ECP. In this context, treatment with SU5402, an inhibitor of fibroblast growth factor (FGF) receptor 1, suppressed Brachyury expression, phosphorylation of ERK1/2, and cardiomyocyte differentiation induced by ECP. We concluded that ECP might induce mesoderm differentiation through FGF signaling pathway and enhance subsequent cardiomyocyte differentiation in concert with dimethyl sulfoxide in P19CL6 cells. ECP may be a novel factor for cardiomyocyte differentiation, which should be very useful to prepare adequate numbers of cardiomyocytes for therapeutic cell transplantation.
巻・号 30(5)
ページ 344-55
公開日 2012-10-1
DOI 10.3109/08977194.2012.709852
PMID 22845717
MeSH Animals Cell Differentiation Cell Line, Tumor Dimethyl Sulfoxide / pharmacology Embryonal Carcinoma Stem Cells / cytology* Embryonal Carcinoma Stem Cells / metabolism Eosinophil Cationic Protein / metabolism* Extracellular Signal-Regulated MAP Kinases / metabolism Fetal Proteins / biosynthesis GATA4 Transcription Factor / biosynthesis Mice Myocytes, Cardiac / cytology* Myocytes, Cardiac / metabolism* Myosin Heavy Chains / biosynthesis Phosphorylation Protein-Tyrosine Kinases / antagonists & inhibitors Pyrroles / pharmacology Receptors, Fibroblast Growth Factor / antagonists & inhibitors Receptors, Fibroblast Growth Factor / metabolism* Signal Transduction T-Box Domain Proteins / biosynthesis
IF 1.543
引用数 2
WOS 分野 ENDOCRINOLOGY & METABOLISM CELL BIOLOGY
リソース情報
ヒト・動物細胞 P19CL6