RRC ID 44021
Author Miyato H, Tsuno NH, Kitayama J.
Title Semaphorin 3C is involved in the progression of gastric cancer.
Journal Cancer Sci.
Abstract Malignant tumors are often associated with denervation, suggesting the functional implication of axonal guidance molecules in tumor growth. Here, we assessed the role of semaphorin 3C (sema3C) in the progression of gastric cancer. Immunohistochemistry of human samples revealed that sema3C was strongly expressed in neoplastic cells, especially at the invasion front. Stable transfection of target sequences of sema3C miRNA did not affect the in vitro proliferative activity of human gastric cancer AZ-521 cells. However, when the tumor growth was examined in vivo using an orthotopic model in nude mice, primary stomach tumors as well as metastatic liver tumors were significantly suppressed by sema3C silencing with the reduction of microvessel density. Immunostaining of primary tumor indicated the rate of Ki-67 positive carcinoma cells was decreased, whereas that of apoptotic cells was significantly increased in sema3C-silenced tumor. In addition, capillary-like tubular formation was reduced by the addition of culture media of sema3C miRNA cells compared with the media of control miRNA cells. Semaphorin 3C is positively expressed in gastric cancer cells and may be involved in tumor progression, presumably through the stimulation of angiogenesis.
Volume 103(11)
Pages 1961-6
Published 2012-11
DOI 10.1111/cas.12003
PMID 22924992
MeSH Animals Apoptosis / genetics Cell Growth Processes / physiology Cell Line, Tumor Disease Progression Endothelial Cells / metabolism Endothelial Cells / pathology Humans Mice Mice, Inbred BALB C Mice, Nude MicroRNAs / genetics Neovascularization, Pathologic / genetics Neovascularization, Pathologic / metabolism Neovascularization, Pathologic / pathology Neuropilin-2 / genetics Neuropilin-2 / metabolism Semaphorins / genetics Semaphorins / metabolism* Stomach Neoplasms / blood supply Stomach Neoplasms / genetics Stomach Neoplasms / metabolism* Stomach Neoplasms / pathology* Transfection / methods
IF 4.751
Times Cited 23
Human and Animal Cells