RRC ID 44064
著者 Hoshino T, Fujita K, Higashi A, Sakiyama K, Ohno H, Morishima K.
タイトル Contracting cardiomyocytes in hydrophobic room-temperature ionic liquid.
ジャーナル Biochem Biophys Res Commun
Abstract Room-temperature ionic liquids (RTILs) are drawing attention as a new class of nonaqueous solvents to replace organic and aqueous solvents for chemical processes in the liquid phase at room temperature. The RTILs are notable for their characteristics of nonvolatility, extremely low vapor pressure, electric conductivity, and incombustibility. These distinguished properties of RTILs have brought attention to them in applications with biological cells and tissue in vacuum environment for scanning electron microscopy, and in microfluidic devices for micro-total analysis system (micro-TAS). Habitable RTILs could increase capability of nonaqueous micro-TAS for living cells. Some RTILs seemed to have the capability to replace water in biological applications. However, these RTILs had been applied to just supplemental additives for biocompatible test, to fixed cells as a substitute for an aqueous solution, and to simple molecules. None of RTILs in which directly soaks a living cell culture. Therefore, we demonstrated the design of RTILs for a living cell culture and a liquid electrolyte to stimulate contracting cardiomyocytes using the RTILs. We assessed the effect of RTILs on the cardiomyocytes using the beating lifetime to compare the applicability of RTILs for biological applications. Frequent spontaneous contractions of cardiomyocytes were confirmed in amino acid anion RTILs [P(8,8,8,8)][Leu] and [P(8,8,8,8)][Ala], phosphoric acid derivatives [P(8,8,8,8)][MeO(H)PO(2)], and [P(8,8,8,8)][C(7)CO(2)]. The anion type of RTILs had influence on applicable characteristics for the contracting cardiomyocyte. This result suggested the possibility for biocompatible design of hydrophobic group RTILs to achieve biological applications with living cells.
巻・号 427(2)
ページ 379-84
公開日 2012-10-19
DOI 10.1016/j.bbrc.2012.09.068
PII S0006-291X(12)01808-6
PMID 23000154
MeSH Alanine / chemistry Alanine / pharmacology Animals Cell Culture Techniques Cells, Cultured Electrolytes / chemistry Electrolytes / pharmacology Heart Rate Hydrophobic and Hydrophilic Interactions Ionic Liquids / chemistry Ionic Liquids / pharmacology* Leucine / chemistry Leucine / pharmacology Myocardial Contraction / drug effects* Myocardial Contraction / physiology Myocytes, Cardiac / drug effects* Myocytes, Cardiac / physiology Phosphoric Acids / chemistry Phosphoric Acids / pharmacology Rats Temperature
IF 2.985
引用数 1
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞