論文 - 詳細
| RRC ID | 44068 |
|---|---|
| 著者 | Mikami T, Koyama S, Yabuta Y, Kitagawa H. |
| タイトル | Chondroitin sulfate is a crucial determinant for skeletal muscle development/regeneration and improvement of muscular dystrophies. |
| ジャーナル | J Biol Chem |
| Abstract |
Skeletal muscle formation and regeneration require myoblast fusion to form multinucleated myotubes or myofibers, yet their molecular regulation remains incompletely understood. We show here that the levels of extra- and/or pericellular chondroitin sulfate (CS) chains in differentiating C2C12 myoblast culture are dramatically diminished at the stage of extensive syncytial myotube formation. Forced down-regulation of CS, but not of hyaluronan, levels enhanced myogenic differentiation in vitro. This characteristic CS reduction seems to occur through a cell-autonomous mechanism that involves HYAL1, a known catabolic enzyme for hyaluronan and CS. In vivo injection of a bacterial CS-degrading enzyme boosted myofiber regeneration in a mouse cardiotoxin-induced injury model and ameliorated dystrophic pathology in mdx muscles. Our data suggest that the control of CS abundance is a promising new therapeutic approach for the treatment of skeletal muscle injury and progressive muscular dystrophies. |
| 巻・号 | 287(46) |
| ページ | 38531-42 |
| 公開日 | 2012-11-9 |
| DOI | 10.1074/jbc.M111.336925 |
| PII | S0021-9258(20)62319-5 |
| PMID | 23007393 |
| PMC | PMC3493898 |
| MeSH | Animals Cardiotoxins / metabolism Cell Differentiation Cells, Cultured Chondroitin Sulfates / chemistry Chondroitin Sulfates / physiology* Gene Expression Regulation* Glycosaminoglycans / metabolism Hyaluronic Acid / chemistry Hyaluronoglucosaminidase / metabolism* Mice Mice, Inbred C57BL Mice, Inbred mdx Muscle Development Muscle, Skeletal / metabolism* Muscular Dystrophies / physiopathology* Muscular Dystrophies / therapy Proteoglycans / metabolism Regeneration Time Factors |
| IF | 4.238 |
| 引用数 | 18 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |