RRC ID 4408
Author Mihara H, Hidese R, Yamane M, Kurihara T, Esaki N.
Title The iscS gene deficiency affects the expression of pyrimidine metabolism genes.
Journal Biochem Biophys Res Commun
Abstract Inactivation of iscS encoding cysteine desulfurase results in a slow growth phenotype associated with the deficiency of iron-sulfur clusters, thiamine, NAD, and tRNA thionucleosides in Escherichia coli. However, the other roles of iscSin vivo are unknown. By using differential screening strategies, we identified 2 pyrimidine salvage enzymes, namely, uridine phosphorylase and cytidine deaminase, which were down-regulated in the iscS mutant. Both enzymes are positively regulated by the cAMP receptor protein (CRP). We also identified a novel protein complex, namely, YeiT-YeiA, whose expression level was decreased in the iscS mutant. The recombinant YeiT-YeiA complex exhibited NADH-dependent dihydropyrimidine dehydrogenase activity, indicating its role in pyrimidine metabolism. The presence of a CRP-binding consensus sequence on the 5'-upstream of the yeiT-YeiA gene suggests its regulation by CRP. These results provide a clue to the possible role of iscS in pyrimidine metabolism by gene regulation.
Volume 372(3)
Pages 407-11
Published 2008-8-1
DOI 10.1016/j.bbrc.2008.05.019
PII S0006-291X(08)00882-6
PMID 18482579
MeSH Base Sequence Carbon-Sulfur Lyases / genetics Carbon-Sulfur Lyases / physiology* Consensus Sequence Cyclic AMP Receptor Protein / metabolism Cytidine Deaminase / genetics Cytidine Deaminase / metabolism Dihydrouracil Dehydrogenase (NAD+) / genetics Dihydrouracil Dehydrogenase (NAD+) / metabolism Dihydrouracil Dehydrogenase (NADP) / metabolism Down-Regulation Electrophoresis, Gel, Two-Dimensional Escherichia coli / genetics* Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Escherichia coli Proteins / physiology* Gene Expression Regulation, Bacterial* Mutation Pyrimidines / metabolism* Transcription Factors / metabolism Uridine Phosphorylase / genetics Uridine Phosphorylase / metabolism
IF 2.985
Times Cited 10
Prokaryotes E. coli MC4100 MG1655 W3110 JW2514 JW1670 JW2781