論文 - 詳細
| RRC ID | 4408 |
|---|---|
| 著者 | Mihara H, Hidese R, Yamane M, Kurihara T, Esaki N. |
| タイトル | The iscS gene deficiency affects the expression of pyrimidine metabolism genes. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Inactivation of iscS encoding cysteine desulfurase results in a slow growth phenotype associated with the deficiency of iron-sulfur clusters, thiamine, NAD, and tRNA thionucleosides in Escherichia coli. However, the other roles of iscSin vivo are unknown. By using differential screening strategies, we identified 2 pyrimidine salvage enzymes, namely, uridine phosphorylase and cytidine deaminase, which were down-regulated in the iscS mutant. Both enzymes are positively regulated by the cAMP receptor protein (CRP). We also identified a novel protein complex, namely, YeiT-YeiA, whose expression level was decreased in the iscS mutant. The recombinant YeiT-YeiA complex exhibited NADH-dependent dihydropyrimidine dehydrogenase activity, indicating its role in pyrimidine metabolism. The presence of a CRP-binding consensus sequence on the 5'-upstream of the yeiT-YeiA gene suggests its regulation by CRP. These results provide a clue to the possible role of iscS in pyrimidine metabolism by gene regulation. |
| 巻・号 | 372(3) |
| ページ | 407-11 |
| 公開日 | 2008-8-1 |
| DOI | 10.1016/j.bbrc.2008.05.019 |
| PII | S0006-291X(08)00882-6 |
| PMID | 18482579 |
| MeSH | Base Sequence Carbon-Sulfur Lyases / genetics Carbon-Sulfur Lyases / physiology* Consensus Sequence Cyclic AMP Receptor Protein / metabolism Cytidine Deaminase / genetics Cytidine Deaminase / metabolism Dihydrouracil Dehydrogenase (NAD+) / genetics Dihydrouracil Dehydrogenase (NAD+) / metabolism Dihydrouracil Dehydrogenase (NADP) / metabolism Down-Regulation Electrophoresis, Gel, Two-Dimensional Escherichia coli / genetics* Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Escherichia coli Proteins / physiology* Gene Expression Regulation, Bacterial* Mutation Pyrimidines / metabolism* Transcription Factors / metabolism Uridine Phosphorylase / genetics Uridine Phosphorylase / metabolism |
| IF | 2.985 |
| 引用数 | 10 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | MC4100 MG1655 W3110 JW2514 JW1670 JW2781 |