論文 - 詳細
| RRC ID | 44081 |
|---|---|
| 著者 | Mie M, Kaneko M, Henmi F, Kobatake E. |
| タイトル | Induction of motor neuron differentiation by transduction of Olig2 protein. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Olig2 protein, a member of the basic helix-loop-helix transcription factor family, was introduced into the mouse embryonic carcinoma cell line P19 for induction of motor neuron differentiation. We show that Olig2 protein has the ability to permeate the cell membrane without the addition of a protein transduction domain (PTD), similar to other basic helix-loop-helix transcription factors such as MyoD and NeuroD2. Motor neuron differentiation was evaluated for the elongation of neurites and the expression of choline acetyltransferase (ChAT) mRNA, a differentiation marker of motor neurons. By addition of Olig2 protein, motor neuron differentiation was induced in P19 cells. |
| 巻・号 | 427(3) |
| ページ | 531-6 |
| 公開日 | 2012-10-26 |
| DOI | 10.1016/j.bbrc.2012.09.090 |
| PII | S0006-291X(12)01837-2 |
| PMID | 23022191 |
| MeSH | Animals Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism* Basic Helix-Loop-Helix Transcription Factors / pharmacology Cell Line, Tumor Choline O-Acetyltransferase / biosynthesis Mice Motor Neurons / cytology* Motor Neurons / drug effects Motor Neurons / metabolism Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Nerve Tissue Proteins / pharmacology Neurites / metabolism Neurites / physiology Neurogenesis / drug effects Neurogenesis / physiology* Oligodendrocyte Transcription Factor 2 Protein Structure, Tertiary Protein Transport RNA, Messenger / biosynthesis |
| IF | 2.985 |
| 引用数 | 7 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |