論文 - 詳細
| RRC ID | 44123 |
|---|---|
| 著者 | Lee J, Miyanaga Y, Ueda M, Hohng S. |
| タイトル | Video-rate confocal microscopy for single-molecule imaging in live cells and superresolution fluorescence imaging. |
| ジャーナル | Biophys J |
| Abstract |
There is no confocal microscope optimized for single-molecule imaging in live cells and superresolution fluorescence imaging. By combining the swiftness of the line-scanning method and the high sensitivity of wide-field detection, we have developed a, to our knowledge, novel confocal fluorescence microscope with a good optical-sectioning capability (1.0 μm), fast frame rates (<33 fps), and superior fluorescence detection efficiency. Full compatibility of the microscope with conventional cell-imaging techniques allowed us to do single-molecule imaging with a great ease at arbitrary depths of live cells. With the new microscope, we monitored diffusion motion of fluorescently labeled cAMP receptors of Dictyostelium discoideum at both the basal and apical surfaces and obtained superresolution fluorescence images of microtubules of COS-7 cells at depths in the range 0-85 μm from the surface of a coverglass. |
| 巻・号 | 103(8) |
| ページ | 1691-7 |
| 公開日 | 2012-10-17 |
| DOI | 10.1016/j.bpj.2012.09.014 |
| PII | S0006-3495(12)01027-2 |
| PMID | 23083712 |
| PMC | PMC3475383 |
| MeSH | Animals COS Cells Chlorocebus aethiops Dictyostelium Fluorescence Resonance Energy Transfer Microscopy, Confocal / instrumentation Microscopy, Confocal / methods* Microscopy, Fluorescence / instrumentation Microscopy, Fluorescence / methods* Microtubules / chemistry Optical Imaging / instrumentation Optical Imaging / methods* Receptors, Cyclic AMP / analysis |
| IF | 3.854 |
| 引用数 | 20 |
| WOS 分野 | BIOPHYSICS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |