RRC ID 44141
著者 Yang Y, Toy W, Choong LY, Hou P, Ashktorab H, Smoot DT, Yeoh KG, Lim YP.
タイトル Discovery of SLC3A2 cell membrane protein as a potential gastric cancer biomarker: implications in molecular imaging.
ジャーナル J Proteome Res
Abstract Despite decreasing incidence and mortality, gastric cancer remains the second leading cause of cancer-related deaths in the world. Successful management of gastric cancer is hampered by lack of highly sensitive and specific biomarkers especially for early cancer detection. Cell surface proteins that are aberrantly expressed between normal and cancer cells are potentially useful for cancer imaging and therapy due to easy accessibility of these targets. Combining two-phase partition and isobaric tags for relative and absolute quantification methods, we compared the relative expression levels of membrane proteins between noncancer and gastric cancer cells. About 33% of the data set was found to be plasma membrane and associated proteins using this approach (compared to only 11% in whole cell analysis), several of which have never been previously implicated in gastric cancer. Upregulation of SLC3A2 in gastric cancer cells was validated by immunoblotting of a panel of 13 gastric cancer cell lines and immunohistochemistry on tissue microarrays comprising 85 matched pairs of normal and tumor tissues. Immunofluorescence and immunohistochemistry both confirmed the plasma membrane localization of SLC3A2 in gastric cancer cells. The data supported the notion that SLC3A2 is a potential biomarker that could be exploited for molecular imaging-based detection of gastric cancer.
巻・号 11(12)
ページ 5736-47
公開日 2012-12-7
DOI 10.1021/pr300555y
PMID 23116296
PMC PMC3749780
MeSH Biomarkers, Tumor / analysis* Biomarkers, Tumor / metabolism Cell Line, Tumor Cell Membrane / metabolism Fluorescent Antibody Technique Fusion Regulatory Protein 1, Heavy Chain / analysis* Fusion Regulatory Protein 1, Heavy Chain / metabolism Humans Immunohistochemistry Isoelectric Focusing Membrane Proteins / analysis Membrane Proteins / metabolism* Molecular Imaging / methods* Neoplasm Proteins / analysis Neoplasm Proteins / metabolism Proteomics / methods Stomach Neoplasms / diagnosis* Stomach Neoplasms / metabolism Tissue Array Analysis
IF 4.074
引用数 40
WOS 分野 BIOCHEMICAL RESEARCH METHODS
リソース情報
ヒト・動物細胞