| RRC ID |
44147
|
| 著者 |
Danjoh I, Shirota R, Hiroyama T, Nakamura Y.
|
| タイトル |
Dominant expansion of a cryptic subclone with an abnormal karyotype in B lymphoblastoid cell lines during culture.
|
| ジャーナル |
Cytogenet Genome Res
|
| Abstract |
Although B lymphoblastoid cell lines (B-LCLs) are thought to maintain their original genomic structures during long-term culture, there has been considerable disagreement on the actual genomic stability of these cells. This study was initiated to determine whether B-LCLs develop cell populations with abnormal genomes during culture and to search for factors important to the maintenance of the original genome. We established continuous cultures of B-LCLs for more than 6 months and analyzed the cells using array-based comparative genome hybridization (CGH) analysis, conventional karyotyping and analysis of V(D)J recombination in the immunoglobulin (Ig) gene. We found that one B-LCL acquired an extra chromosome 4 without any other genomic rearrangements at passage 16 of continuous culture. At the Ig light- and heavy-chain loci, analysis of the major cell population showed a difference between cultures at early and later passages. Another aneuploid line was detected among B-LCLs established elsewhere and deposited previously into the RIKEN Cell Bank. Our findings indicate that some of the genomic rearrangements in B-LCLs are not caused by gradual accumulation of mutations and rearrangements during the B-LCL establishment processes, but rather as a result of a change in the cell population from clones with a normal genome to clones with de novo rearrangements. It is therefore feasible to maintain B-LCLs with a normal genomic structure by cell cloning or similar treatment.
|
| 巻・号 |
139(2)
|
| ページ |
88-96
|
| 公開日 |
2013-1-1
|
| DOI |
10.1159/000343757
|
| PII |
000343757
|
| PMID |
23128794
|
| MeSH |
Abnormal Karyotype*
Adult
Aneuploidy
B-Lymphocytes / cytology
B-Lymphocytes / metabolism*
Cell Line
Cell Proliferation*
Cells, Cultured
Chromosomes, Human, Pair 4 / genetics*
Clone Cells / cytology
Clone Cells / metabolism
Comparative Genomic Hybridization
Genes, Immunoglobulin / genetics
Humans
Infant, Newborn
Karyotype
Time Factors
V(D)J Recombination
|
| IF |
1.114
|
| 引用数 |
3
|
|
WOS 分野
|
GENETICS & HEREDITY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RPMI1788(RCB1878) |
