論文 - 詳細
| RRC ID | 44175 |
|---|---|
| 著者 | Akiyama I, Yoshino O, Osuga Y, Izumi G, Urata Y, Hirota Y, Hirata T, Harada M, Koga K, Ogawa K, Kozuma S. |
| タイトル | Follistatin is induced by IL-1β and TNF-α in stromal cells from endometrioma. |
| ジャーナル | Reprod Sci |
| Abstract |
The aim of this study is to examine the regulation of follistatin, an activin-binding protein, in endometriosis. Endometrioma stromal cells (EoSCs) were obtained from 9 patients undergoing laparoscopy of the ovarian endometrioma. In cultured EoSCs, interleukin 1β (IL-1β) and tumor necrosis factor-α (TNF-α), which could induce activin-A, also induced follistatin messenger RNA (mRNA) and protein. The cystic fluid of endometrioma from 8 patients was obtained to measure the concentration of activin-A and follistatin by enzyme-linked immunosorbent assay (ELISA). Also, activin activity in the fluid was examined by erythroid differentiation assay using mouse erythroleukemia F5-5.fl cells. In the cystic fluid of endometrioma, the mean values of activin-A and follistatin concentration were 36.8 ng/mL and 4.0 ng/mL, respectively. In a bioassay, all 8 samples exhibited activin activity, which was equivalent to recombinant activin-A activity of 12.8 ± 1.4 ng/mL. Although follistatin was present in the cystic fluid of endometrioma, the activity of activin, which is an exacerbation factor of endometriosis, was predominant in vivo. |
| 巻・号 | 20(6) |
| ページ | 675-9 |
| 公開日 | 2013-6-1 |
| DOI | 10.1177/1933719112463253 |
| PII | 1933719112463253 |
| PMID | 23171678 |
| MeSH | Activins / metabolism Animals Cell Line Endometriosis / metabolism* Enzyme-Linked Immunosorbent Assay Female Follistatin / genetics Follistatin / metabolism* Humans Interleukin-1beta / metabolism* Mice RNA, Messenger / metabolism Real-Time Polymerase Chain Reaction Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Stromal Cells / metabolism* Tumor Necrosis Factor-alpha / metabolism* Up-Regulation |
| IF | 2.616 |
| 引用数 | 2 |
| WOS 分野 | OBSTETRICS & GYNECOLOGY REPRODUCTIVE BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | F5-5.fl(RCB0560) |