論文 - 詳細
| RRC ID | 44181 |
|---|---|
| 著者 | Mogi A, Koga K, Aoki M, Hamasaki M, Uesugi N, Iwasaki A, Shirakusa T, Tamura K, Nabeshima K. |
| タイトル | Expression and role of GLUT-1, MCT-1, and MCT-4 in malignant pleural mesothelioma. |
| ジャーナル | Virchows Arch |
| Abstract |
Malignant cells supply their energy needs through increased glucose consumption, producing large quantities of lactic acid via glycolysis. Glucose transporters (GLUTs) and monocarboxylate transporters (MCTs) are therefore commonly up-regulated in human malignancies to mediate glucose influx and lactic acid efflux, respectively. However, their roles in malignant pleural mesothelioma (MPM) have not been fully elucidated. Here, we evaluated GLUT-1, MCT-1, and MCT-4 expression in human MPM and reactive mesothelial hyperplasia (RMH) and elucidated their biological role in vitro. GLUT-1, MCT-1, and MCT-4 expression was determined in human MPM (n = 35) and RMH (n = 20) specimens by immunohistochemistry and in frozen tissue, and MPM cell lines, by real-time reverse transcription-polymerase chain reaction and western blot analysis. GLUT-1, MCT-1, and MCT-4 functions in MPM were evaluated by transfection with small interfering RNA. Immunohistochemical analysis revealed higher levels of GLUT-1, MCT-1, and MCT-4 in MPM than in RMH. Additionally, GLUT-1, MCT-1, and MCT-4 mRNA levels were higher in MPM than in non-neoplastic mesothelial cell lines. The siRNA-mediated knockdown of GLUT-1 or MCT-1 significantly suppressed tumor cell proliferation, and MCT-1 silencing inhibited invasion and induced apoptosis. Taken together, these results indicate that combined application of GLUT-1, MCT-1, and MCT-4 immunohistochemistry might be useful in differentiating MPM from RMH and suggest that MCT-1plays an important biological role. |
| 巻・号 | 462(1) |
| ページ | 83-93 |
| 公開日 | 2013-1-1 |
| DOI | 10.1007/s00428-012-1344-6 |
| PMID | 23187830 |
| MeSH | Apoptosis Biomarkers, Tumor / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Line, Tumor Cell Proliferation Diagnosis, Differential Epithelium / pathology Female Gene Expression Regulation, Neoplastic / physiology Gene Knockdown Techniques Gene Silencing Glucose Transporter Type 1 / genetics Glucose Transporter Type 1 / metabolism* Humans Hyperplasia / diagnosis Lung Neoplasms / genetics Lung Neoplasms / metabolism* Lung Neoplasms / pathology Lung Neoplasms / surgery Male Mesothelioma / genetics Mesothelioma / metabolism* Mesothelioma / pathology Mesothelioma / surgery Mesothelioma, Malignant Middle Aged Monocarboxylic Acid Transporters / genetics Monocarboxylic Acid Transporters / metabolism* Muscle Proteins / genetics Muscle Proteins / metabolism* Oncogene Proteins / genetics Oncogene Proteins / metabolism* Pleural Neoplasms / genetics Pleural Neoplasms / metabolism* Pleural Neoplasms / pathology Pleural Neoplasms / surgery RNA, Small Interfering / genetics Transfection |
| IF | 2.906 |
| 引用数 | 11 |
| WOS 分野 | PATHOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ACC-MESO-1(RCB2292) |