RRC ID |
44206
|
Author |
Hogerheyde TA, Stephenson SA, Harkin DG, Bray LJ, Madden PW, Woolf MI, Richardson NA.
|
Title |
Evaluation of Eph receptor and ephrin expression within the human cornea and limbus.
|
Journal |
Exp Eye Res
|
Abstract |
Eph receptor tyrosine kinases and their ligands, the ephrins, regulate the development and maintenance of multiple organs but little is known about their potential role within the cornea. The purpose of this study was to perform a thorough investigation of Eph/ephrin expression within the human cornea including the limbal stem cell niche. Initially, immunohistochemistry was performed on human donor eyes to determine the spatial distribution of Eph receptors and ephrins in the cornea and limbus. Patterns of Eph/ephrin gene expression in (1) immortalised human corneal endothelial (B4G12) or corneal epithelial (HCE-T) cell lines, and (2) primary cultures of epithelial or stromal cells established from the corneal limbus of cadaveric eye tissue were then assessed by reverse transcription (RT) PCR. Limbal epithelial or stromal cells from primary cultures were also assessed for evidence of Eph/ephrin-reactivity by immunofluorescence. Immunoreactivity for ephrinA1 and EphB4 was detected in the corneal endothelium of donor eyes. EphB4 was also consistently detected in the limbal and corneal epithelium and in cells located in the stroma of the peripheral cornea. Expression of multiple Eph/ephrin genes was detected in immortalised corneal epithelial and endothelial cell lines. Evidence of Eph/ephrin gene expression was also demonstrated in primary cultures of human limbal stromal (EphB4, B6; ephrinA5) and epithelial cells (EphA1, A2; ephrinA5, B2) using both RT-PCR and immunofluorescence. The expression of Eph receptors and ephrins within the human cornea and limbus is much wider than previously appreciated and suggests multiple potential roles for these molecules in the maintenance of normal corneal architecture.
|
Volume |
107
|
Pages |
110-20
|
Published |
2013-2-1
|
DOI |
10.1016/j.exer.2012.11.016
|
PII |
S0014-4835(12)00343-0
|
PMID |
23247085
|
MeSH |
Cells, Cultured
Cornea / metabolism*
DNA Primers / chemistry
Endothelium, Corneal / metabolism
Ephrins / genetics*
Ephrins / metabolism
Epithelium, Corneal / metabolism
Fluorescent Antibody Technique, Indirect
Gene Expression Regulation / physiology*
Humans
Immunoenzyme Techniques
Limbus Corneae / metabolism*
Polymerase Chain Reaction
RNA, Messenger / metabolism
Receptors, Eph Family / genetics*
Receptors, Eph Family / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tissue Donors
|
IF |
3.011
|
Times Cited |
7
|
WOS Category
|
OPHTHALMOLOGY
|
Resource |
Human and Animal Cells |
HCE-T(RCB2280) |