Reference - Detail
| RRC ID | 44293 |
|---|---|
| Author | Liu Y, Sakai S, Taya M. |
| Title | Impact of the composition of alginate and gelatin derivatives in bioconjugated hydrogels on the fabrication of cell sheets and spherical tissues with living cell sheaths. |
| Journal | Acta Biomater |
| Abstract |
Gelatin and alginate derivatives possessing phenolic hydroxyl moieties (gelatin-Ph and Alg-Ph) were dissolved in aqueous solution and conjugated via horseradish peroxidase-catalyzed crosslinking, resulting in hydrogelation. The objective of creating the hydrogels was to prepare cell sheets and spherical tissues wrapped in living cell sheaths. An increase in the gelatin-Ph content in the hydrogel improved cellular adhesion on the hydrogel surface but hindered degradability by alginate lyase. A hydrogel with the desired characteristics was obtained from a solution containing 0.5% (w/v) gelatin-Ph and 1.5% (w/v) Alg-Ph. Human aortic endothelial (HAE) cells and mouse embryo fibroblast 10T1/2 cells grew on the hydrogels and could be harvested as cell sheets by treatment with alginate lyase. 10T1/2 cells enclosed in Alg-Ph/gelatin-Ph microcapsules composed of the conjugate hydrogel elongated on the inner surface of the microcapsules and grew three times faster than those enclosed in Alg-Ph microcapsules. Alg-Ph/gelatin-Ph microcapsules not only supported growth of the enclosed cells into spherical tissues, but also provided a cell adhesive outer surface for the fabrication of an HAE cell layer. Finally, spherical tissues of 10T1/2 cells wrapped in living HAE cell sheaths were obtained by treatment with alginate lyase. |
| Volume | 9(5) |
| Pages | 6616-23 |
| Published | 2013-5-1 |
| DOI | 10.1016/j.actbio.2013.01.037 |
| PII | S1742-7061(13)00052-4 |
| PMID | 23395920 |
| MeSH | Alginates / administration & dosage* Alginates / chemistry Animals Cell Adhesion Cell Proliferation Cells, Cultured Gelatin / administration & dosage* Gelatin / chemistry Glucuronic Acid / administration & dosage Glucuronic Acid / chemistry Hexuronic Acids / administration & dosage Hexuronic Acids / chemistry Humans Hydrogels* Mice Polysaccharide-Lyases / administration & dosage Tissue Scaffolds* |
| IF | 7.242 |
| Times Cited | 24 |
| WOS Category | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| Resource | |
| Human and Animal Cells | |