論文 - 詳細
RRC ID | 44293 |
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著者 | Liu Y, Sakai S, Taya M. |
タイトル | Impact of the composition of alginate and gelatin derivatives in bioconjugated hydrogels on the fabrication of cell sheets and spherical tissues with living cell sheaths. |
ジャーナル | Acta Biomater |
Abstract |
Gelatin and alginate derivatives possessing phenolic hydroxyl moieties (gelatin-Ph and Alg-Ph) were dissolved in aqueous solution and conjugated via horseradish peroxidase-catalyzed crosslinking, resulting in hydrogelation. The objective of creating the hydrogels was to prepare cell sheets and spherical tissues wrapped in living cell sheaths. An increase in the gelatin-Ph content in the hydrogel improved cellular adhesion on the hydrogel surface but hindered degradability by alginate lyase. A hydrogel with the desired characteristics was obtained from a solution containing 0.5% (w/v) gelatin-Ph and 1.5% (w/v) Alg-Ph. Human aortic endothelial (HAE) cells and mouse embryo fibroblast 10T1/2 cells grew on the hydrogels and could be harvested as cell sheets by treatment with alginate lyase. 10T1/2 cells enclosed in Alg-Ph/gelatin-Ph microcapsules composed of the conjugate hydrogel elongated on the inner surface of the microcapsules and grew three times faster than those enclosed in Alg-Ph microcapsules. Alg-Ph/gelatin-Ph microcapsules not only supported growth of the enclosed cells into spherical tissues, but also provided a cell adhesive outer surface for the fabrication of an HAE cell layer. Finally, spherical tissues of 10T1/2 cells wrapped in living HAE cell sheaths were obtained by treatment with alginate lyase. |
巻・号 | 9(5) |
ページ | 6616-23 |
公開日 | 2013-5-1 |
DOI | 10.1016/j.actbio.2013.01.037 |
PII | S1742-7061(13)00052-4 |
PMID | 23395920 |
MeSH | Alginates / administration & dosage* Alginates / chemistry Animals Cell Adhesion Cell Proliferation Cells, Cultured Gelatin / administration & dosage* Gelatin / chemistry Glucuronic Acid / administration & dosage Glucuronic Acid / chemistry Hexuronic Acids / administration & dosage Hexuronic Acids / chemistry Humans Hydrogels* Mice Polysaccharide-Lyases / administration & dosage Tissue Scaffolds* |
IF | 7.242 |
引用数 | 24 |
WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
リソース情報 | |
ヒト・動物細胞 |