論文 - 詳細
| RRC ID | 44314 |
|---|---|
| 著者 | Uchida F, Uzawa K, Kasamatsu A, Takatori H, Sakamoto Y, Ogawara K, Shiiba M, Bukawa H, Tanzawa H. |
| タイトル | Overexpression of CDCA2 in human squamous cell carcinoma: correlation with prevention of G1 phase arrest and apoptosis. |
| ジャーナル | PLoS One |
| Abstract |
Cell division cycle associated 2 (CDCA2) recruits protein phosphatase 1 to chromatin to antagonize activation of ataxia telangiectasia mutated (ATM)-dependent signal transduction. ATM kinase plays a critical role in the DNA damage response and its phosphorylation cascade to inhibit the p53-MDM2 interaction, which releases p53 to induce p21 and G1 cell-cycle arrest. However, the relevance of CDCA2 to human malignancy including oral squamous cell carcinoma (OSCC) is unknown. In the current study, we found that CDCA2 expression was up-regulated in OSCC cell lines. Functional studies with shRNA system showed that knockdown of CDCA2 significantly (P<0.05) inhibited cellular proliferation compared with the control cells by arresting cell-cycle progression at the G1 phase and up-regulating the cyclin-dependent kinase inhibitors (p21(Cip1), p27(Kip1), p15(INK4B), and p16(INK4A)). CDCA2 knockdown also promoted apoptosis after treatment with the DNA damage reagent, cisplatin. In clinical samples, the CDCA2 protein expression level in primary OSCCs was significantly (P<0.05) greater than in matched normal oral tissues (67/85, 79%). Furthermore, CDCA2-positive cases were correlated significantly (P<0.05) with high cancer progression. Our results showed for the first time that CDCA2 frequently is overexpressed in OSCCs and might be associated closely with OSCC progression by preventing cell-cycle arrest and apoptosis. |
| 巻・号 | 8(2) |
| ページ | e56381 |
| 公開日 | 2013-1-1 |
| DOI | 10.1371/journal.pone.0056381 |
| PII | PONE-D-12-13569 |
| PMID | 23418564 |
| PMC | PMC3572040 |
| MeSH | Aged Antineoplastic Agents / pharmacology Apoptosis / drug effects Apoptosis / genetics Blotting, Western Carcinoma, Squamous Cell / genetics* Carcinoma, Squamous Cell / metabolism Carcinoma, Squamous Cell / pathology Carrier Proteins / genetics* Carrier Proteins / metabolism Cell Cycle Proteins / genetics* Cell Cycle Proteins / metabolism Cell Line, Tumor Cell Proliferation Cisplatin / pharmacology Cyclin-Dependent Kinase Inhibitor p15 / metabolism Cyclin-Dependent Kinase Inhibitor p16 / metabolism Cyclin-Dependent Kinase Inhibitor p21 / metabolism Cyclin-Dependent Kinase Inhibitor p27 / metabolism Female G1 Phase Cell Cycle Checkpoints / genetics Gene Expression Regulation, Neoplastic* HeLa Cells Humans Immunohistochemistry Ki-67 Antigen / metabolism Male Middle Aged Mouth Neoplasms / genetics* Mouth Neoplasms / metabolism Mouth Neoplasms / pathology Nuclear Proteins / genetics* Nuclear Proteins / metabolism RNA Interference Reverse Transcriptase Polymerase Chain Reaction |
| IF | 2.74 |
| 引用数 | 42 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HSC-3(RCB1975) HSC-2(RCB1945) HSC-4(RCB1902) Ca9-22(RCB1976) HO-1-u-1(RCB2102) Sa3(RCB0980) |