RRC ID 44317
著者 Thang NM, Kumasawa K, Tsutsui T, Nakamura H, Masaki H, Ono T, Kimura T.
タイトル Overexpression of endogenous TIMP-2 increases the proliferation of BeWo choriocarcinoma cells through the MAPK-signaling pathway.
ジャーナル Reprod Sci
Abstract Choriocarcinoma is a highly malignant form of trophoblastic tumor that is characterized by malignant placental tumors and rapid cell growth. In vivo and in vitro studies have demonstrated that tissue inhibitor of metalloproteinase 2 (TIMP-2) is present in choriocarcinoma. However, the role of TIMP-2 in cell proliferation in choriocarcinoma has not been investigated. Exogenous TIMP-2 is known to promote cell proliferation. During growth, cells are subjected to varied concentrations of TIMP-2, which depend on the amount of TIMP-2 produced by the cells themselves. Thus, the effect of gradually increasing endogenous TIMP-2 on the proliferation of choriocarcinoma cells needs to be examined. Proliferation of BeWo human choriocarcinoma cells was stimulated by transient transfection of a plasmid expressing TIMP-2. Overexpression of endogenous TIMP-2 also activated ERK1/2 and JNK1/2 of the MAPK-signaling pathway. Furthermore, inhibition of these proteins resulted in suppression of the cell proliferation-stimulating effect of TIMP-2. These results suggest that TIMP-2 plays an important role in tumor growth in the case of BeWo cells. Moreover, proliferation of BeWo cells due to TIMP-2 expression can be used as a model for fast-growing choriocarcinomas, and TIMP-2 could be used as a novel tumor marker of choriocarcinoma.
巻・号 20(10)
ページ 1184-92
公開日 2013-10-1
DOI 10.1177/1933719113477485
PII 1933719113477485
PMID 23427184
MeSH Cell Proliferation* Choriocarcinoma / enzymology* Choriocarcinoma / pathology Gene Expression Regulation, Neoplastic* HEK293 Cells Humans MAP Kinase Signaling System / physiology* Tissue Inhibitor of Metalloproteinase-2 / biosynthesis*
IF 2.616
引用数 8
WOS 分野 OBSTETRICS & GYNECOLOGY REPRODUCTIVE BIOLOGY
リソース情報
ヒト・動物細胞 293T(RCB2202)