論文 - 詳細
| RRC ID | 44334 |
|---|---|
| 著者 | Kito T, Shibata R, Ishii M, Suzuki H, Himeno T, Kataoka Y, Yamamura Y, Yamamoto T, Nishio N, Ito S, Numaguchi Y, Tanigawa T, Yamashita JK, Ouchi N, Honda H, Isobe K, Murohara T. |
| タイトル | iPS cell sheets created by a novel magnetite tissue engineering method for reparative angiogenesis. |
| ジャーナル | Sci Rep |
| Abstract |
Angiogenic cell therapy represents a novel strategy for ischemic diseases, but some patients show poor responses. We investigated the therapeutic potential of an induced pluripotent stem (iPS) cell sheet created by a novel magnetite tissue engineering technology (Mag-TE) for reparative angiogenesis. Mouse iPS cell-derived Flk-1(+) cells were incubated with magnetic nanoparticle-containing liposomes (MCLs). MCL-labeled Flk-1(+) cells were mixed with diluted extracellular matrix (ECM) precursor and a magnet was placed on the reverse side. Magnetized Flk-1(+) cells formed multi-layered cell sheets according to magnetic force. Implantation of the Flk-1(+) cell sheet accelerated revascularization of ischemic hindlimbs relative to the contralateral limbs in nude mice as measured by laser Doppler blood flow and capillary density analyses. The Flk-1(+) cell sheet also increased the expressions of VEGF and bFGF in ischemic tissue. iPS cell-derived Flk-1(+) cell sheets created by this novel Mag-TE method represent a promising new modality for therapeutic angiogenesis. |
| 巻・号 | 3 |
| ページ | 1418 |
| 公開日 | 2013-1-1 |
| DOI | 10.1038/srep01418 |
| PII | srep01418 |
| PMID | 23475393 |
| PMC | PMC3593218 |
| MeSH | Animals Cell Culture Techniques Cells, Cultured Extracellular Matrix / metabolism Fibroblast Growth Factor 2 / metabolism Hindlimb / metabolism Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Induced Pluripotent Stem Cells / transplantation Ischemia / metabolism Ischemia / pathology Ischemia / therapy Laser-Doppler Flowmetry Liposomes / chemistry Magnetite Nanoparticles / chemistry* Mice Mice, Nude Neovascularization, Physiologic Tissue Engineering* Vascular Endothelial Growth Factor A / metabolism |
| IF | 3.998 |
| 引用数 | 33 |
| WOS 分野 | CARDIAC & CARDIOVASCULAR SYSTEMS |
| リソース情報 | |
| ヒト・動物細胞 | iPS-MEF-Ng-20D-17(APS0001) |