RRC ID 4438
Author Johnston PA, Timmerman-Vaughan GM, Farnden KJ, Pickering R.
Title Marker development and characterisation of Hordeum bulbosum introgression lines: a resource for barley improvement.
Journal Theor Appl Genet
Abstract A set of 110 diploid putative introgression lines (ILs) containing chromatin introgressed from the undomesticated species Hordeum bulbosum L. (bulbous barley grass) into cultivated barley (Hordeum vulgare L.) has been identified using a high-copy number retrotransposon-like PCR marker, pSc119.1, derived from rye (Secale cereale L.). To evaluate these lines, 92 EST-derived markers were developed by marker sequencing across four barley cultivars and four H. bulbosum genotypes. Single nucleotide polymorphisms and insertions/deletions conserved between the two species were then used to develop a set of fully informative cleaved amplified polymorphic sequence markers or size polymorphic insertion/deletion markers. Introgressed chromatin from H. bulbosum was confirmed and genetically located in 88 of these lines using 46 of the EST-derived PCR markers. A total of 96 individual introgressions were detected with most of them (94.8%) extending to the most distal marker for each respective chromosome arm. Introgressions were detected on all chromosome arms except chromosome 3HL. Interstitial or sub-distal introgressions also occurred, with two located on chromosome 2HL and one each on 3HS, 5HL and 6HS. Twenty-two putative ILs that were positive for H. bulbosum chromatin using pSc119.1 have not had introgressions detected with these single-locus markers. When all introgressions are combined, more than 36% of the barley genetic map has now been covered with introgressed chromatin from H. bulbosum. These ILs represent a significant germplasm resource for barley improvement that can be mined for diverse traits of interest to barley breeders and researchers.
Volume 118(8)
Pages 1429-37
Published 2009-5-1
DOI 10.1007/s00122-009-0992-7
PMID 19263032
MeSH Alleles Base Sequence Chromatin / genetics Chromosome Mapping Chromosomes, Plant Crops, Agricultural / genetics* DNA, Plant / genetics Diploidy Exons Expressed Sequence Tags Gene Deletion Gene Dosage Genes, Plant Genetic Markers* Genotype Hordeum / genetics* Hordeum / growth & development* Hybridization, Genetic* Introns Molecular Sequence Data Mutagenesis, Insertional Polymorphism, Single Nucleotide Secale / genetics Sequence Homology, Nucleic Acid
IF 4.439
Times Cited 29
Barley Hordeum vulgare? H.bulbosum?