RRC ID 44401
著者 Haque A, Koide N, Odkhuu E, Tsolmongyn B, Naiki Y, Komatsu T, Yoshida T, Yokochi T.
タイトル Mouse pyrin and HIN domain family member 1 (pyhin1) protein positively regulates LPS-induced IFN-β and NO production in macrophages.
ジャーナル Innate Immun
Abstract The pyrin and HIN-domain (PYHIN) family member1 (pyhin1) is a member of PYHIN proteins and involved in transcriptional regulation of genes important for cell cycle control, differentiation and apoptosis. The regulatory action of mouse pyhin1 on LPS-induced inflammatory response was examined. LPS augmented the pyhin1 mRNA expression in murine RAW 264.7 macrophage cells and peritoneal macrophages. The augmentation of pyhin1 mRNA expression was abolished by parthenolide, a NF-κB inhibitor. Silencing of pyhin1 with small interfering RNA reduced the production of IFN-β and NO. However, pyhin1 silencing did not affect the production of TNF-α, IL-6, IL-10 and prostaglandin E2. Reduced IFN-β production by pyhin1 silencing caused inactivation of STAT1 and reduced expression of IRF1. Pyhin1 silencing inhibited the expression of TRAF6, TBK1 and TRIF, which trigger IFN-β production in the MyD88-independent pathway. However, pyhin1 silencing did not affect the expression of MyD88, IRAK4 and several mitogen-activated protein kinases in the MyD88-dependent pathway. Taken together, mouse pyhin1 was suggested to be a NF-κB-responsible gene in response to LPS and positively regulate LPS-induced IFN-β and NO production through up-regulating the MyD88-independent signaling pathway.
巻・号 20(1)
ページ 40-8
公開日 2014-1-1
DOI 10.1177/1753425913481636
PII 1753425913481636
PMID 23606517
MeSH Animals Cell Line Gene Expression Regulation / drug effects Inflammation* / immunology Interferon Regulatory Factor-1 / genetics Interferon Regulatory Factor-1 / metabolism Interferon-beta / metabolism* Lipopolysaccharides / immunology Macrophages, Peritoneal / immunology* Mice NF-kappa B / antagonists & inhibitors Nitric Oxide / metabolism* Nuclear Proteins / genetics Nuclear Proteins / metabolism* RNA, Small Interfering / genetics STAT1 Transcription Factor / genetics STAT1 Transcription Factor / metabolism Sesquiterpenes / pharmacology Signal Transduction / drug effects TNF Receptor-Associated Factor 6 / genetics TNF Receptor-Associated Factor 6 / metabolism
IF 2.298
引用数 8
WOS 分野 MEDICINE, RESEARCH & EXPERIMENTAL BIOCHEMISTRY & MOLECULAR BIOLOGY IMMUNOLOGY MICROBIOLOGY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)