Reference - Detail
RRC ID | 44480 |
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Author | Tsolmongyn B, Koide N, Odkhuu E, Haque A, Naiki Y, Komatsu T, Yoshida T, Yokochi T. |
Title | Lipopolysaccharide prevents valproic acid-induced apoptosis via activation of nuclear factor-κB and inhibition of p53 activation. |
Journal | Cell Immunol |
Abstract |
The effect of lipopolysaccharide (LPS) on valproic acid (VPA)-induced cell death was examined by using mouse RAW 264.7 macrophage cells. LPS inhibited the activation of caspase 3 and poly (ADP-ribose) polymerase and prevented VPA-induced apoptosis. LPS inhibited VPA-induced p53 activation and pifithrin-α as a p53 inhibitor as well as LPS prevented VPA-induced apoptosis. LPS abolished the increase of Bax/Bcl-2 ratio, which is a critical indicator of p53-mediated mitochondrial damage, in response to VPA. The nuclear factor (NF)-κB inhibitors, Bay 11-7082 and parthenolide, abolished the preventive action of LPS on VPA-induced apoptosis. A series of toll-like receptor ligands, Pam3CSK4, poly I:C, and CpG DNA as well as LPS prevented VPA-induced apoptosis. Taken together, LPS was suggested to prevent VPA-induced apoptosis via activation of anti-apoptotic NF-κB and inhibition of pro-apoptotic p53 activation. The detailed inhibitory mechanism of VPA-induced apoptosis by LPS is discussed. |
Volume | 282(2) |
Pages | 100-5 |
Published | 2013-4-1 |
DOI | 10.1016/j.cellimm.2013.04.011 |
PII | S0008-8749(13)00062-2 |
PMID | 23770718 |
MeSH | Animals Apoptosis / drug effects* Cell Line Dose-Response Relationship, Drug Immunoblotting Lipopeptides / pharmacology Lipopolysaccharides / pharmacology* Macrophages / cytology Macrophages / drug effects Macrophages / metabolism Mice NF-kappa B / antagonists & inhibitors NF-kappa B / metabolism* Nitriles / pharmacology Oligodeoxyribonucleotides / pharmacology Poly I-C / pharmacology Proto-Oncogene Proteins c-bcl-2 / metabolism Sesquiterpenes / pharmacology Sulfones / pharmacology Toll-Like Receptor 9 / agonists Toll-Like Receptors / agonists Tumor Suppressor Protein p53 / metabolism* Valproic Acid / pharmacology* bcl-2-Associated X Protein / metabolism |
IF | 4.078 |
Times Cited | 15 |
WOS Category | IMMUNOLOGY CELL BIOLOGY |
Resource | |
Human and Animal Cells | RAW 264(RCB0535) |