| RRC ID |
44486
|
| 著者 |
Tone Y, Kawahara M, Hayashi J, Nagamune T.
|
| タイトル |
Cell fate conversion by conditionally switching the signal-transducing domain of signalobodies.
|
| ジャーナル |
Biotechnol Bioeng
|
| Abstract |
Conditionally and strictly controlling cell fates is important for biomedical applications including cell therapies. Although previous studies have been based on regulating the expression or activation of signaling molecules, the techniques therein require improvement in terms of reducing leakiness and complexity. In this study, we propose a novel cell fate converting system using our previously developed antibody/receptor chimeras named "signalobodies" in combination with a Cre/loxP recombination system. We designed a "switch vector" where a growth signalobody gene was flanked by two loxP sites and a death signalobody gene was placed downstream of the floxed cassette. Cells transduced with the switch vector showed superior growth activity in the presence of a specific antigen. Subsequent expression of Cre induced the death signalobody, leading to conditional cell death. This technology could be applicable for other cell fate conversion systems including differentiation and migration, by using appropriate signal-transducing domains.
|
| 巻・号 |
110(12)
|
| ページ |
3219-26
|
| 公開日 |
2013-12-1
|
| DOI |
10.1002/bit.24985
|
| PMID |
23794462
|
| MeSH |
Antibodies / genetics
Antibodies / metabolism*
Cell Physiological Phenomena*
Cell Survival / drug effects
Cytological Techniques / methods*
Humans
Integrases
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism*
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Recombination, Genetic
Signal Transduction / drug effects*
|
| IF |
4.002
|
| 引用数 |
2
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Ba/F3(RCB0805) |
