論文 - 詳細
| RRC ID | 44488 |
|---|---|
| 著者 | Ito M, Arakawa T, Okayama M, Shitara A, Mizoguchi I, Takuma T. |
| タイトル | Gravity loading induces adenosine triphosphate release and phosphorylation of extracellular signal-regulated kinases in human periodontal ligament cells. |
| ジャーナル | J Investig Clin Dent |
| Abstract |
AIM:The periodontal ligament (PDL) receives mechanical stress (MS) from dental occlusion or orthodontic tooth movement. Mechanical stress is thought to be a trigger for remodeling of the PDL and alveolar bone, although its signaling mechanism is still unclear. So we investigated the effect of MS on adenosine triphosphate (ATP) release and extracellular signal-regulated kinases (ERK) phosphorylation in PDL cells. METHODS:Mechanical stress was applied to human PDL cells as centrifugation-mediated gravity loading. Apyrase, Ca(2+)-free medium and purinergic receptor agonists and antagonists were utilized to analyze the contribution of purinergic receptors to ERK phosphorylation. RESULTS:Gravity loading and ATP increased ERK phosphorylation by 5 and 2.5 times, respectively. Gravity loading induced ATP release from PDL cells by tenfold. Apyrase and suramin diminished ERK phosphorylation induced by both gravity loading and ATP. Under Ca(2+)-free conditions the phosphorylation by gravity loading was partially decreased, whereas ATP-induced phosphorylation was unaffected. Receptors P2Y4 and P2Y6 were prominently expressed in the PDL cells. CONCLUSION:Gravity loading induced ATP release and ERK phosphorylation in PDL fibroblasts, and ATP signaling via P2Y receptors was partially involved in this phosphorylation, which in turn would enhance gene expression for the remodeling of PDL tissue during orthodontic tooth movement. |
| 巻・号 | 5(4) |
| ページ | 266-74 |
| 公開日 | 2014-11-1 |
| DOI | 10.1111/jicd.12049 |
| PMID | 23798356 |
| MeSH | Adenosine Diphosphate / analogs & derivatives Adenosine Diphosphate / pharmacology Adenosine Triphosphate / antagonists & inhibitors Adenosine Triphosphate / metabolism* Apyrase / pharmacology Calcium / pharmacology Cell Culture Techniques Cells, Cultured Centrifugation Extracellular Signal-Regulated MAP Kinases / drug effects Extracellular Signal-Regulated MAP Kinases / metabolism* Fibroblasts / drug effects Fibroblasts / metabolism Gingiva / cytology Gingiva / drug effects Gingiva / metabolism Gravitation Humans Periodontal Ligament / cytology Periodontal Ligament / drug effects Periodontal Ligament / metabolism* Phosphorylation Purinergic Agonists / pharmacology Purinergic Antagonists / pharmacology Purinergic P2X Receptor Antagonists / pharmacology Purinergic P2Y Receptor Antagonists / pharmacology Receptors, Purinergic P2 / drug effects Signal Transduction / drug effects Stress, Mechanical Suramin / pharmacology |
| 引用数 | 9 |
| リソース情報 | |
| ヒト・動物細胞 | Saos-2(RCB0428) |