RRC ID 44489
Author Kawamoto Y, Ohyama Y, Chiba T, Yagishita H, Sakashita H, Imai K.
Title Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-associated lymphoid tissue 1 expression.
Journal Int J Oncol
Abstract Progression of oral carcinomas associates with aberrant activation and inactivation of molecules that work in established or unknown pathways. Although mucosa‑associated lymphoid tissue 1 (MALT1) expressed in normal oral epithelium is inactivated in the aggressive subset of carcinomas with worse prognosis, phenotypic changes of carcinoma cells upon the loss of expression is unknown. We performed a proteomic analysis to identify MALT1‑regulated proteins in oral carcinoma cells. Four different keratins were included in the ten most abundantly changed proteins. K8/18 were upregulated in MALT1 stably‑expressing carcinoma cells and K5/14 in MALT1‑marginal control cells. K8/18 upregulation and K5/14 downregulation were MALT1 dose‑dependent and observed in a series of oral carcinoma cells. MALT1 suppressed cell proliferation (0.52-fold, P<0.01) and its dominant-negative form stimulated it (1.33-fold, P<0.01). The decreased proliferation associated with reduction of cyclin D1, which was recovered by the short interfering RNA against MALT1. Taken together, loss of MALT1 expression alters keratin expression and enhances proliferation of carcinoma cells, and may progress oral carcinomas into the advanced state.
Volume 43(3)
Pages 729-36
Published 2013-9-1
DOI 10.3892/ijo.2013.1990
PMID 23799590
MeSH Carcinoma / genetics* Carcinoma / pathology Caspases / biosynthesis Caspases / genetics* Cell Line, Tumor Cell Proliferation Cyclin D1 / biosynthesis Epithelium / metabolism Epithelium / pathology Gene Expression Regulation, Neoplastic Humans Keratins / biosynthesis* Keratins / genetics Keratins / metabolism Mouth Neoplasms / genetics* Mouth Neoplasms / pathology Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein Mucous Membrane / metabolism Mucous Membrane / pathology Neoplasm Proteins / biosynthesis Neoplasm Proteins / genetics* Proteomics / methods Up-Regulation
IF 3.899
Times Cited 4
Human and Animal Cells HSC-2(RCB1945) HSC-3(RCB1975) Ca9-22(RCB1976)