| RRC ID |
44489
|
| 著者 |
Kawamoto Y, Ohyama Y, Chiba T, Yagishita H, Sakashita H, Imai K.
|
| タイトル |
Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-associated lymphoid tissue 1 expression.
|
| ジャーナル |
Int J Oncol
|
| Abstract |
Progression of oral carcinomas associates with aberrant activation and inactivation of molecules that work in established or unknown pathways. Although mucosa‑associated lymphoid tissue 1 (MALT1) expressed in normal oral epithelium is inactivated in the aggressive subset of carcinomas with worse prognosis, phenotypic changes of carcinoma cells upon the loss of expression is unknown. We performed a proteomic analysis to identify MALT1‑regulated proteins in oral carcinoma cells. Four different keratins were included in the ten most abundantly changed proteins. K8/18 were upregulated in MALT1 stably‑expressing carcinoma cells and K5/14 in MALT1‑marginal control cells. K8/18 upregulation and K5/14 downregulation were MALT1 dose‑dependent and observed in a series of oral carcinoma cells. MALT1 suppressed cell proliferation (0.52-fold, P<0.01) and its dominant-negative form stimulated it (1.33-fold, P<0.01). The decreased proliferation associated with reduction of cyclin D1, which was recovered by the short interfering RNA against MALT1. Taken together, loss of MALT1 expression alters keratin expression and enhances proliferation of carcinoma cells, and may progress oral carcinomas into the advanced state.
|
| 巻・号 |
43(3)
|
| ページ |
729-36
|
| 公開日 |
2013-9-1
|
| DOI |
10.3892/ijo.2013.1990
|
| PMID |
23799590
|
| MeSH |
Carcinoma / genetics*
Carcinoma / pathology
Caspases / biosynthesis
Caspases / genetics*
Cell Line, Tumor
Cell Proliferation
Cyclin D1 / biosynthesis
Epithelium / metabolism
Epithelium / pathology
Gene Expression Regulation, Neoplastic
Humans
Keratins / biosynthesis*
Keratins / genetics
Keratins / metabolism
Mouth Neoplasms / genetics*
Mouth Neoplasms / pathology
Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein
Mucous Membrane / metabolism
Mucous Membrane / pathology
Neoplasm Proteins / biosynthesis
Neoplasm Proteins / genetics*
Proteomics / methods
Up-Regulation
|
| IF |
3.899
|
| 引用数 |
4
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HSC-2(RCB1945)
HSC-3(RCB1975)
Ca9-22(RCB1976) |
