論文 - 詳細
RRC ID | 44493 |
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著者 | Fujiya A, Nagasaki H, Seino Y, Okawa T, Kato J, Fukami A, Himeno T, Uenishi E, Tsunekawa S, Kamiya H, Nakamura J, Oiso Y, Hamada Y. |
タイトル | The role of S100B in the interaction between adipocytes and macrophages. |
ジャーナル | Obesity (Silver Spring) |
Abstract |
OBJECTIVE:The S100 calcium binding protein B (S100B) implicated in brain inflammation acts via the receptor of advanced glycation end products (RAGE) and is also secreted from adipocytes. We investigated the role of S100B in the interaction between adipocytes and macrophages using a cell-culture model. DESIGN AND METHODS:RAW264.7 macrophages (RAW) were stimulated by recombinant S100B to observe alterations in TNF-α and M1 markers; 3T3-L1 adipocytes (L1) were stimulated by TNF-α to examine S100B secretion. RAW and L1 were then mutually stimulated with conditioned media of each other, or co-cultured. The effects of S100B silencing or a RAGE-neutralizing antibody were also investigated. RESULTS:S100B upregulated TNF-α and M1 markers in RAW, and TNF-α augmented S100B secretion from L1. L1 conditioned media stimulated TNF-α secretion from RAW, and RAW conditioned media increased S100B secretion from L1. The co-culture of RAW and L1 increased TNF-α, S100B, and the expression of M1 markers and the MCP-1 receptor CCR2. The silencing of S100B or RAGE neutralization significantly ameliorated TNF-α hypersecretion from RAW that were stimulated with L1 conditioned media. CONCLUSIONS:Thus, S100B as an adipokine may play a role in the interaction between adipocytes and macrophages to establish a vicious paracrine loop. |
巻・号 | 22(2) |
ページ | 371-9 |
公開日 | 2014-2-1 |
DOI | 10.1002/oby.20532 |
PMID | 23804363 |
MeSH | 3T3-L1 Cells Adipocytes, White / drug effects Adipocytes, White / immunology Adipocytes, White / metabolism* Adipokines / antagonists & inhibitors Adipokines / genetics Adipokines / metabolism Animals Antibodies, Neutralizing / pharmacology Cell Communication* / drug effects Cell Line, Transformed Coculture Techniques Culture Media, Conditioned / metabolism Gene Silencing Immunomodulation / drug effects Insulin Resistance Macrophage Activation / drug effects Macrophages / drug effects Macrophages / immunology Macrophages / metabolism* Mice Obesity / immunology Obesity / metabolism Paracrine Communication / drug effects Receptor for Advanced Glycation End Products Receptors, Immunologic / agonists* Receptors, Immunologic / antagonists & inhibitors Receptors, Immunologic / metabolism Recombinant Proteins / chemistry Recombinant Proteins / metabolism S100 Calcium Binding Protein beta Subunit / antagonists & inhibitors S100 Calcium Binding Protein beta Subunit / genetics S100 Calcium Binding Protein beta Subunit / metabolism* Signal Transduction* / drug effects Tumor Necrosis Factor-alpha / agonists Tumor Necrosis Factor-alpha / metabolism* |
IF | 3.742 |
引用数 | 30 |
WOS 分野 | NUTRITION & DIETETICS ENDOCRINOLOGY & METABOLISM |
リソース情報 | |
ヒト・動物細胞 | RAW 264(RCB0535) |