| RRC ID |
44499
|
| Author |
Miyashita S, Sagane Y, Niwa K, Watanabe T.
|
| Title |
Transport of the botulinum neurotoxin-associating protein, nontoxic nonhemagglutinin, across the rat small intestinal epithelial cell monolayer.
|
| Journal |
FEMS Microbiol Lett
|
| Abstract |
Botulinum neurotoxin (BoNT) associates with nontoxic nonhemagglutinin (NTNHA) yielding a complex in culture. BoNT and NTNHA have similar domain organizations, implying that they share common functions, although this remains unclear. Here, we examined cell monolayer transport of serotype D NTNHA in the rat intestinal epithelial cell line IEC-6. NTNHA and BoNT both bound to the cell and were transported across the cell layer. NTNHA contains a QXW motif and a β-trefoil fold, both common in sugar chain-recognizing proteins, whereas the QXW motif is absent in all BoNT serotypes. This could explain the distinct sugar chain-recognizing properties of NTNHA and BoNT.
|
| Volume |
346(1)
|
| Pages |
73-80
|
| Published |
2013-9-1
|
| DOI |
10.1111/1574-6968.12205
|
| PMID |
23809018
|
| MeSH |
Amino Acid Motifs
Amino Acid Sequence
Animals
Bacterial Proteins / metabolism*
Botulinum Toxins / metabolism
Cell Line
Epithelial Cells / metabolism*
Microscopy, Confocal
Microscopy, Fluorescence
Models, Molecular
Molecular Sequence Data
Protein Binding
Protein Conformation
Protein Transport
Rats
Sequence Alignment
|
| IF |
1.987
|
| Times Cited |
4
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| Human and Animal Cells |
IEC 6(RCB0993) |
