論文 - 詳細
| RRC ID | 44499 |
|---|---|
| 著者 | Miyashita S, Sagane Y, Niwa K, Watanabe T. |
| タイトル | Transport of the botulinum neurotoxin-associating protein, nontoxic nonhemagglutinin, across the rat small intestinal epithelial cell monolayer. |
| ジャーナル | FEMS Microbiol Lett |
| Abstract |
Botulinum neurotoxin (BoNT) associates with nontoxic nonhemagglutinin (NTNHA) yielding a complex in culture. BoNT and NTNHA have similar domain organizations, implying that they share common functions, although this remains unclear. Here, we examined cell monolayer transport of serotype D NTNHA in the rat intestinal epithelial cell line IEC-6. NTNHA and BoNT both bound to the cell and were transported across the cell layer. NTNHA contains a QXW motif and a β-trefoil fold, both common in sugar chain-recognizing proteins, whereas the QXW motif is absent in all BoNT serotypes. This could explain the distinct sugar chain-recognizing properties of NTNHA and BoNT. |
| 巻・号 | 346(1) |
| ページ | 73-80 |
| 公開日 | 2013-9-1 |
| DOI | 10.1111/1574-6968.12205 |
| PMID | 23809018 |
| MeSH | Amino Acid Motifs Amino Acid Sequence Animals Bacterial Proteins / metabolism* Botulinum Toxins / metabolism Cell Line Epithelial Cells / metabolism* Microscopy, Confocal Microscopy, Fluorescence Models, Molecular Molecular Sequence Data Protein Binding Protein Conformation Protein Transport Rats Sequence Alignment |
| IF | 1.987 |
| 引用数 | 4 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | IEC 6(RCB0993) |