論文 - 詳細
| RRC ID | 44512 |
|---|---|
| 著者 | Spiess C, Merchant M, Huang A, Zheng Z, Yang NY, Peng J, Ellerman D, Shatz W, Reilly D, Yansura DG, Scheer JM. |
| タイトル | Bispecific antibodies with natural architecture produced by co-culture of bacteria expressing two distinct half-antibodies. |
| ジャーナル | Nat Biotechnol |
| Abstract |
By enabling the simultaneous engagement of two distinct targets, bispecific antibodies broaden the potential utility of antibody-based therapies. However, bispecific-antibody design and production remain challenging, owing to the need to incorporate two distinct heavy and light chain pairs while maintaining natural nonimmunogenic antibody architecture. Here we present a bispecific-antibody production strategy that relies on co-culture of two bacterial strains, each expressing a half-antibody. Using this approach, we produce 28 unique bispecific antibodies. A bispecific antibody against the receptor tyrosine kinases MET and EGFR binds both targets monovalently, inhibits their signaling, and suppresses MET and EGFR-driven cell and tumor growth. Our strategy allows rapid generation of bispecific antibodies from any two existing antibodies and yields milligram to gram quantities of bispecific antibodies sufficient for a wide range of discovery and preclinical applications. |
| 巻・号 | 31(8) |
| ページ | 753-8 |
| 公開日 | 2013-8-1 |
| DOI | 10.1038/nbt.2621 |
| PII | nbt.2621 |
| PMID | 23831709 |
| MeSH | Antibodies, Bispecific / biosynthesis* Antibodies, Bispecific / immunology Antibody Specificity Bacteria / immunology Bacteria / metabolism Cell Line, Tumor Coculture Techniques* ErbB Receptors / genetics ErbB Receptors / immunology* Gene Expression Regulation, Bacterial / immunology Humans Immunoglobulin G / genetics Immunoglobulin G / immunology Neoplasms / immunology Neoplasms / pathology Neoplasms / therapy* Protein Engineering Proto-Oncogene Proteins c-met / genetics Proto-Oncogene Proteins c-met / immunology* |
| IF | 36.553 |
| 引用数 | 113 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 325 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 9.0 |
| リソース情報 | |
| ヒト・動物細胞 | KP4(RCB1005) |