| RRC ID |
44516
|
| 著者 |
Takenaka Y, Inoue I, Nakano T, Shinoda Y, Ikeda M, Awata T, Katayama S.
|
| タイトル |
A Novel Splicing Variant of Peroxisome Proliferator-Activated Receptor-γ (Pparγ1sv) Cooperatively Regulates Adipocyte Differentiation with Pparγ2.
|
| ジャーナル |
PLoS One
|
| Abstract |
Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors that regulate expression of a number of genes associated with the cellular differentiation and development. Here, we show the abundant and ubiquitous expression of a newly identified splicing variant of mouse Pparγ (Pparγ1sv) that encodes PPARγ1 protein, and its importance in adipogenesis. The novel splicing variant has a unique 5'-UTR sequence, relative to those of Pparγ1 and Pparγ2 mRNAs, indicating the presence of a novel transcriptional initiation site and promoter for Pparγ expression. Pparγ1sv was highly expressed in the white and brown adipose tissues at levels comparable to Pparγ2. Pparγ1sv was synergistically up-regulated with Pparγ2 during adipocyte differentiation of 3T3-L1 cells and mouse primary cultured preadipocytes. Inhibition of Pparγ1sv by specific siRNAs completely abolished the induced adipogenesis in 3T3-L1 cells. C/EBPβ and C/EBPδ activated both the Pparγ1sv and Pparγ2 promoters in 3T3-L1 preadipocytes. These findings suggest that Pparγ1sv and Pparγ2 synergistically regulate the early stage of the adipocyte differentiation.
|
| 巻・号 |
8(6)
|
| ページ |
e65583
|
| 公開日 |
2013-1-1
|
| DOI |
10.1371/journal.pone.0065583
|
| PII |
PONE-D-12-38283
|
| PMID |
23840343
|
| PMC |
PMC3686765
|
| MeSH |
3T3-L1 Cells
5' Untranslated Regions
Adipocytes / cytology*
Adipocytes / metabolism
Adipogenesis*
Adipose Tissue, Brown / metabolism
Adipose Tissue, White / metabolism
Alternative Splicing
Animals
CCAAT-Enhancer-Binding Protein-beta / metabolism*
CCAAT-Enhancer-Binding Protein-delta / metabolism*
Cell Differentiation
Cells, Cultured
Mice
PPAR gamma / genetics*
PPAR gamma / metabolism*
Promoter Regions, Genetic
RNA, Messenger / metabolism
Tissue Distribution
Up-Regulation
|
| IF |
2.74
|
| 引用数 |
9
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
ST2(RCB0224) |
