Reference - Detail
| RRC ID | 44539 |
|---|---|
| Author | Tanaka M, Kishimoto KN, Okuno H, Saito H, Itoi E. |
| Title | Vitamin D receptor gene silencing effects on differentiation of myogenic cell lines. |
| Journal | Muscle Nerve |
| Abstract |
INTRODUCTION:The active form of vitamin D (1,25-dihydroxy-vitamin D3) is known to increase fast-type myosin heavy chain expression in differentiated myogenic cell lines. The mechanisms for this effect are not fully understood. The aim of this study was to determine the role of signals transmitted through the vitamin D receptor (VDR) during differentiation of myoblasts. METHODS:Electroporation was used to introduce VDR siRNA molecules into C2C12 and G8 murine myoblast cell lines. Gene and protein expression profiles of VDR-gene silenced cells were analyzed in vitro. RESULTS:Suppressing VDR expression by RNA interference resulted in inhibition of myogenic differentiation of C2C12 and G8 cell lines at both mRNA and protein levels. CONCLUSIONS:Our results suggest that myoblasts require signals transmitted through VDR for differentiation into myocytes and emphasize the importance of VDR expression in skeletal muscles for maintaining muscle volume in the elderly. |
| Volume | 49(5) |
| Pages | 700-8 |
| Published | 2014-5-1 |
| DOI | 10.1002/mus.23950 |
| PMID | 23873355 |
| MeSH | Animals Cell Differentiation / genetics Cell Differentiation / physiology* Cell Line Gene Silencing* Mice Muscle Cells / metabolism Muscle Cells / physiology Muscle Development / genetics Muscle Development / physiology* Myoblasts / metabolism* Myogenin / genetics Myogenin / metabolism Myosin Heavy Chains / genetics Myosin Heavy Chains / metabolism RNA, Small Interfering Receptors, Calcitriol / genetics Receptors, Calcitriol / physiology* Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / physiology* |
| IF | 2.505 |
| Times Cited | 20 |
| WOS Category | CLINICAL NEUROLOGY NEUROSCIENCES |
| Resource | |
| Human and Animal Cells | C2C12(RCB0987) |