論文 - 詳細
| RRC ID | 44584 |
|---|---|
| 著者 | Yoshitaka T, Kawai A, Miyaki S, Numoto K, Kikuta K, Ozaki T, Lotz M, Asahara H. |
| タイトル | Analysis of microRNAs expressions in chondrosarcoma. |
| ジャーナル | J Orthop Res |
| Abstract |
MicroRNAs (miRNAs) are small non-coding RNAs capable of inhibiting gene expression post-transcriptionally and expression profiling can provide therapeutic targets and tools for cancer diagnosis. Chondrosarcoma is a mesenchymal tumor with unknown cause and differentiation status. Here, we profiled miRNA expression of chondrosarcoma, namely clinical samples from human conventional chondrosarcoma tissue, established chondrosarcoma cell lines, and primary non-tumorous adult articular chondrocytes, by miRNA array and quantitative real-time PCR. A wide variety of miRNAs were differently downregulated in chondrosarcoma compared to non-tumorous articular chondrocytes; 27 miRNAs: miR-10b, 23b, 24-1*, 27b, 100, 134, 136, 136*, 138, 181d, 186, 193b, 221*, 222, 335, 337-5p, 376a, 376a*, 376b, 376c, 377, 454, 495, 497, 505, 574-3p, and 660, were significantly downregulated in chondrosarcoma and only 2: miR-96 and 183, were upregulated. We further validated the expression levels of miRNAs by quantitative real-time PCR for miR-181a, let-7a, 100, 222, 136, 376a, and 335 in extended number of chondrosarcoma clinical samples. Among them, all except miR-181a were found to be significantly downregulated in chondrosarcoma derived samples. The findings provide potential diagnostic value and new molecular understanding of chondrosarcoma. |
| 巻・号 | 31(12) |
| ページ | 1992-8 |
| 公開日 | 2013-12-1 |
| DOI | 10.1002/jor.22457 |
| PMID | 23940002 |
| PMC | PMC3835201 |
| MeSH | Adult Aged Aged, 80 and over Bone Neoplasms / genetics* Cell Line, Tumor Chondrosarcoma / genetics* Female Gene Expression Profiling Gene Expression Regulation, Neoplastic HEK293 Cells Humans Male MicroRNAs / analysis* Middle Aged |
| IF | 2.728 |
| 引用数 | 51 |
| WOS 分野 | ORTHOPEDICS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |