論文 - 詳細
| RRC ID | 44591 |
|---|---|
| 著者 | Irie T, Yoshida A, Sakaguchi T. |
| タイトル | Clustered basic amino acids of the small sendai virus C protein Y1 are critical to its RAN GTPase-mediated nuclear localization. |
| ジャーナル | PLoS One |
| Abstract |
The Sendai virus (SeV) C proteins are shown to exert multiple functions during the course of infection. Perhaps reflecting their many functions, they occur at multiple sites of the cell. In this study, we focused on the nuclear-localizing ability of the smaller C protein, Y1, and found that this translocation is mediated by Ran GTPase but not by passive diffusion, and that basic residues within the 149-157 amino acid region are critical for that. The mechanism of inhibition of interferon (IFN)-signaling seemed to differ between the C and Y1 proteins, since deletion of 12 C-terminal amino acids resulted in a loss of the function for the C but not for the Y1 protein. The ability of Y1 mutants to inhibit IFN-α-induced, ISRE-driven expression of a reporter gene almost paralleled with that to localize in the nucleus. These results suggest that nuclear localization of the Y1 protein might be important for the inhibitory effect on type-I IFN-stimulated gene expression. |
| 巻・号 | 8(8) |
| ページ | e73740 |
| 公開日 | 2013-1-1 |
| DOI | 10.1371/journal.pone.0073740 |
| PII | PONE-D-13-18785 |
| PMID | 23951363 |
| PMC | PMC3739745 |
| MeSH | Active Transport, Cell Nucleus Amino Acids, Basic / chemistry Amino Acids, Basic / metabolism* Cell Nucleus / metabolism Cell Nucleus / ultrastructure Cell Nucleus / virology* Gene Expression Regulation Genes, Reporter Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism HEK293 Cells Host-Pathogen Interactions Humans Interferon Regulatory Factor-3 / genetics Interferon Regulatory Factor-3 / metabolism Interferon-alpha / genetics Interferon-alpha / metabolism Phosphorylation Sendai virus / genetics* Sendai virus / metabolism Signal Transduction Viral Proteins / chemistry Viral Proteins / genetics* Viral Proteins / metabolism ran GTP-Binding Protein / genetics* ran GTP-Binding Protein / metabolism |
| IF | 2.74 |
| 引用数 | 7 |
| WOS 分野 | VIROLOGY |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |