論文 - 詳細
| RRC ID | 44659 |
|---|---|
| 著者 | Tanigawa T, Odkhuu E, Morikawa A, Hayashi K, Sato T, Shibata R, Goto F, Ueda H, Yokochi T. |
| タイトル | Immunological role of prostaglandin E2 production in mouse auditory cells in response to LPS. |
| ジャーナル | Innate Immun |
| Abstract |
The effect of LPS on the production of prostaglandin E2 (PGE2) in mouse HEI-OC1 auditory cells was examined. HEI-OC1 auditory cells constitutively produce a small amount of PGE2. LPS augmented the PGE2 production via enhanced cyclooxygenase 2 (COX2) expression. LPS-induced augmentation of COX2 expression was dependent on up-regulation of COX2 mRNA expression. LPS induced the production of TNF-α, but not IL-1β· An anti-TNF-α neutralizing Ab significantly inhibited PGE2 production and COX2 mRNA expression in response to LPS. LPS-induced PGE2 production was prevented by a series of pharmacological signaling inhibitors to NF-κB and MAPKs. Pam3CSK4 as a TLR2 ligand, as well as LPS as a TLR4 ligand, augmented the PGE2 production. However, poly I:C as a TLR3 ligand, imiquimod as a TLR7 ligand and CpG DNA as a TLR9 ligand did not augment it. HEI-OC1 cells expressed TLR2, TLR4 and TLR9, but not TLR3 or TLR7. The putative role of LPS-induced PGE2 production in auditory cells is discussed. |
| 巻・号 | 20(6) |
| ページ | 639-46 |
| 公開日 | 2014-8-1 |
| DOI | 10.1177/1753425913503578 |
| PII | 1753425913503578 |
| PMID | 24055878 |
| MeSH | Animals Cell Line Cochlea / cytology Cochlea / immunology* Cyclooxygenase 2 / biosynthesis Dinoprostone / immunology* Interleukin-1beta / biosynthesis Lipopolysaccharides / pharmacology* Mice Mitogen-Activated Protein Kinases / antagonists & inhibitors NF-kappa B / antagonists & inhibitors RNA, Messenger / biosynthesis RNA, Messenger / genetics Toll-Like Receptor 2 / biosynthesis Toll-Like Receptor 4 / biosynthesis Toll-Like Receptor 9 / biosynthesis Tumor Necrosis Factor-alpha / biosynthesis Up-Regulation / drug effects |
| IF | 2.298 |
| 引用数 | 2 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL BIOCHEMISTRY & MOLECULAR BIOLOGY IMMUNOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |