| RRC ID |
44659
|
| 著者 |
Tanigawa T, Odkhuu E, Morikawa A, Hayashi K, Sato T, Shibata R, Goto F, Ueda H, Yokochi T.
|
| タイトル |
Immunological role of prostaglandin E2 production in mouse auditory cells in response to LPS.
|
| ジャーナル |
Innate Immun
|
| Abstract |
The effect of LPS on the production of prostaglandin E2 (PGE2) in mouse HEI-OC1 auditory cells was examined. HEI-OC1 auditory cells constitutively produce a small amount of PGE2. LPS augmented the PGE2 production via enhanced cyclooxygenase 2 (COX2) expression. LPS-induced augmentation of COX2 expression was dependent on up-regulation of COX2 mRNA expression. LPS induced the production of TNF-α, but not IL-1β· An anti-TNF-α neutralizing Ab significantly inhibited PGE2 production and COX2 mRNA expression in response to LPS. LPS-induced PGE2 production was prevented by a series of pharmacological signaling inhibitors to NF-κB and MAPKs. Pam3CSK4 as a TLR2 ligand, as well as LPS as a TLR4 ligand, augmented the PGE2 production. However, poly I:C as a TLR3 ligand, imiquimod as a TLR7 ligand and CpG DNA as a TLR9 ligand did not augment it. HEI-OC1 cells expressed TLR2, TLR4 and TLR9, but not TLR3 or TLR7. The putative role of LPS-induced PGE2 production in auditory cells is discussed.
|
| 巻・号 |
20(6)
|
| ページ |
639-46
|
| 公開日 |
2014-8-1
|
| DOI |
10.1177/1753425913503578
|
| PII |
1753425913503578
|
| PMID |
24055878
|
| MeSH |
Animals
Cell Line
Cochlea / cytology
Cochlea / immunology*
Cyclooxygenase 2 / biosynthesis
Dinoprostone / immunology*
Interleukin-1beta / biosynthesis
Lipopolysaccharides / pharmacology*
Mice
Mitogen-Activated Protein Kinases / antagonists & inhibitors
NF-kappa B / antagonists & inhibitors
RNA, Messenger / biosynthesis
RNA, Messenger / genetics
Toll-Like Receptor 2 / biosynthesis
Toll-Like Receptor 4 / biosynthesis
Toll-Like Receptor 9 / biosynthesis
Tumor Necrosis Factor-alpha / biosynthesis
Up-Regulation / drug effects
|
| IF |
2.298
|
| 引用数 |
2
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
BIOCHEMISTRY & MOLECULAR BIOLOGY
IMMUNOLOGY
MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
