RRC ID 44659
Author Tanigawa T, Odkhuu E, Morikawa A, Hayashi K, Sato T, Shibata R, Goto F, Ueda H, Yokochi T.
Title Immunological role of prostaglandin E2 production in mouse auditory cells in response to LPS.
Journal Innate Immun
Abstract The effect of LPS on the production of prostaglandin E2 (PGE2) in mouse HEI-OC1 auditory cells was examined. HEI-OC1 auditory cells constitutively produce a small amount of PGE2. LPS augmented the PGE2 production via enhanced cyclooxygenase 2 (COX2) expression. LPS-induced augmentation of COX2 expression was dependent on up-regulation of COX2 mRNA expression. LPS induced the production of TNF-α, but not IL-1β· An anti-TNF-α neutralizing Ab significantly inhibited PGE2 production and COX2 mRNA expression in response to LPS. LPS-induced PGE2 production was prevented by a series of pharmacological signaling inhibitors to NF-κB and MAPKs. Pam3CSK4 as a TLR2 ligand, as well as LPS as a TLR4 ligand, augmented the PGE2 production. However, poly I:C as a TLR3 ligand, imiquimod as a TLR7 ligand and CpG DNA as a TLR9 ligand did not augment it. HEI-OC1 cells expressed TLR2, TLR4 and TLR9, but not TLR3 or TLR7. The putative role of LPS-induced PGE2 production in auditory cells is discussed.
Volume 20(6)
Pages 639-46
Published 2014-8-1
DOI 10.1177/1753425913503578
PII 1753425913503578
PMID 24055878
MeSH Animals Cell Line Cochlea / cytology Cochlea / immunology* Cyclooxygenase 2 / biosynthesis Dinoprostone / immunology* Interleukin-1beta / biosynthesis Lipopolysaccharides / pharmacology* Mice Mitogen-Activated Protein Kinases / antagonists & inhibitors NF-kappa B / antagonists & inhibitors RNA, Messenger / biosynthesis RNA, Messenger / genetics Toll-Like Receptor 2 / biosynthesis Toll-Like Receptor 4 / biosynthesis Toll-Like Receptor 9 / biosynthesis Tumor Necrosis Factor-alpha / biosynthesis Up-Regulation / drug effects
IF 2.173
Times Cited 2
Human and Animal Cells