論文 - 詳細
| RRC ID | 44694 |
|---|---|
| 著者 | Nishimori M, Yakushiji H, Mori M, Miyamoto T, Yaguchi T, Ohno S, Miyake Y, Sakaguchi T, Ueda M, Ohno E. |
| タイトル | Tumorigenesis in cells derived from induced pluripotent stem cells. |
| ジャーナル | Hum Cell |
| Abstract |
Induced pluripotent stem (iPS) cells are an attractive source for potential cell-replacement therapy. However, transplantation of differentiated products harbors the risk of teratoma formation, presenting a serious health risk. Thus, we characterized Nanog-expressing (undifferentiated) cells remaining after induction of differentiation by cytological examination. To induce differentiation of iPS cells, we generated embryoid bodies (EBs) derived from iPS cells carrying a Nanog–green fluorescent protein(GFP) reporter and then injected GFP-positive and GFP negative EBs into nude mice. GFP-positive EB transplantation resulted in the formation of immature teratoma grade 3, but no tumors were induced by GFP-negative EB. GFP positive cells revealed significantly lower cytoplasmic area and higher nucleus/cytoplasm ratio than those of GFP negative cells. Our results suggest that morphological analysis might be a useful method for distinguishing between tumorigenic and nontumorigenic iPS cells. |
| 巻・号 | 27(1) |
| ページ | 29-35 |
| 公開日 | 2014-1-1 |
| DOI | 10.1007/s13577-013-0078-3 |
| PMID | 24122447 |
| MeSH | Animals Cell Differentiation Cell Transformation, Neoplastic* Cells, Cultured Embryoid Bodies / cytology Green Fluorescent Proteins Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / pathology* Mice Mice, Nude Stem Cell Transplantation / adverse effects* Teratoma / etiology* Teratoma / pathology* |
| IF | 3.463 |
| 引用数 | 22 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | iPS-MEF-Ng-20D-17(APS0001) |