| RRC ID |
44712
|
| 著者 |
Ito T, Kajino K, Abe M, Sato K, Maekawa H, Sakurada M, Orita H, Wada R, Kajiyama Y, Hino O.
|
| タイトル |
ERC/mesothelin is expressed in human gastric cancer tissues and cell lines.
|
| ジャーナル |
Oncol Rep
|
| Abstract |
ERC/mesothelin is expressed in mesothelioma and other malignancies. The ERC/mesothelin gene (MSLN) encodes a 71-kDa precursor protein, which is cleaved to yield 31-kDa N-terminal (N-ERC/mesothelin) and 40-kDa C-terminal (C-ERC/mesothelin) proteins. N-ERC/mesothelin is a soluble protein and has been reported to be a diagnostic serum marker of mesothelioma and ovarian cancer. Gastric cancer tissue also expresses C-ERC/mesothelin, but the significance of serum N-ERC levels for diagnosing gastric cancer has not yet been studied. We examined the latter issue in the present study as well as C-ERC/mesothelin expression in human gastric cancer tissues and cell lines. We immunohistochemically examined C-ERC/mesothelin expression in tissue samples from 50 cases of gastric cancer, and we also assessed the C-ERC/mesothelin expression in 6 gastric cancer cell lines (MKN-1, MKN-7, MKN-74, NUGC-3, NUGC-4 and TMK-1) using reverse transcription-polymerase chain reaction, flow cytometry, immunohistochemistry and immunoblotting. We also examined the N-ERC/mesothelin concentrations in the supernatants of cultured cells and in the sera of gastric cancer patients using an enzyme-linked immunosorbent assay (ELISA). N-ERC/mesothelin was detected in the supernatants of 3 gastric cancer cell lines (MKN-1, NUGC-4 and TMK-1) by ELISA, but its concentration in the sera of gastric cancer patients was almost same as that observed in the sera of the normal controls. In the gastric cancer tissues, C-ERC/mesothelin expression was associated with lymphatic invasion. N-ERC/mesothelin was secreted into the supernatants of gastric cancer cell lines, but does not appear to be a useful serum marker of gastric cancer.
|
| 巻・号 |
31(1)
|
| ページ |
27-33
|
| 公開日 |
2014-1-1
|
| DOI |
10.3892/or.2013.2803
|
| PMID |
24146039
|
| PMC |
PMC3868502
|
| MeSH |
Adult
Aged
Aged, 80 and over
Biomarkers, Tumor / biosynthesis*
Biomarkers, Tumor / blood
Biomarkers, Tumor / genetics
Cell Line, Tumor
Female
GPI-Linked Proteins / biosynthesis*
GPI-Linked Proteins / blood
GPI-Linked Proteins / genetics
Gene Expression
Humans
Lymphatic Metastasis
Male
Mesothelin
Middle Aged
Stomach Neoplasms / blood
Stomach Neoplasms / genetics
Stomach Neoplasms / metabolism*
|
| IF |
3.417
|
| 引用数 |
10
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MKN1(RCB1003)
MKN7(RCB0999)
MKN74(RCB1002)
NUGC-4(RCB1939) |
