RRC ID 44881
Author Kumeta M, Gilmore JL, Umeshima H, Ishikawa M, Kitajiri S, Horigome T, Kengaku M, Takeyasu K.
Title Caprice/MISP is a novel F-actin bundling protein critical for actin-based cytoskeletal reorganizations.
Journal Genes Cells
Abstract Caprice [C19orf21 actin-bundling protein in characteristic epithelial cells, also called mitotic interactor and substrate of Plk1 (MISP)] is a novel actin-related protein identified in the highly-insoluble subcellular scaffold proteins. This protein contains multiple actin-binding sites, forms characteristic mesh-like F-actin bundles in vitro, and exhibits capricious localization and expression patterns in vivo. Overexpression or knock-down of Caprice resulted in a dramatic effect on cellular morphology by inducing stress fiber-like thick filaments or filopodial formations, respectively. Caprice is expressed and localized in distinct cells and tissues with specialized actin-based structures, such as growth cones of migrating neurons and stereocilia of inner ear hair cells. However, Caprice gene expression is varied among different cell types; especially enriched in several epithelial cells whereas relatively suppressed in a subset of epithelial cells, fibroblasts, and neuroblastoma cells at the transcriptional level. Thus, this protein is expected to be an effector for cell type-specific actin reorganization with its direct actin-binding properties and provides a novel model of cell morphology regulation by a non-ubiquitous single actin-bundling protein.
Volume 19(4)
Pages 338-49
Published 2014-4-1
DOI 10.1111/gtc.12131
PMID 24475924
MeSH Actin Cytoskeleton / metabolism* Actin Cytoskeleton / ultrastructure Actins / metabolism* Animals Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cells, Cultured Dogs Humans Mice Microfilament Proteins / genetics Microfilament Proteins / metabolism* Phosphoproteins / genetics Phosphoproteins / metabolism* Protein Binding Protein Interaction Domains and Motifs Pseudopodia / metabolism
IF 1.655
Times Cited 5
Human and Animal Cells A549 DU145(RCB2143) MDCK(RCB0995) NB1