| RRC ID |
44881
|
| 著者 |
Kumeta M, Gilmore JL, Umeshima H, Ishikawa M, Kitajiri S, Horigome T, Kengaku M, Takeyasu K.
|
| タイトル |
Caprice/MISP is a novel F-actin bundling protein critical for actin-based cytoskeletal reorganizations.
|
| ジャーナル |
Genes Cells
|
| Abstract |
Caprice [C19orf21 actin-bundling protein in characteristic epithelial cells, also called mitotic interactor and substrate of Plk1 (MISP)] is a novel actin-related protein identified in the highly-insoluble subcellular scaffold proteins. This protein contains multiple actin-binding sites, forms characteristic mesh-like F-actin bundles in vitro, and exhibits capricious localization and expression patterns in vivo. Overexpression or knock-down of Caprice resulted in a dramatic effect on cellular morphology by inducing stress fiber-like thick filaments or filopodial formations, respectively. Caprice is expressed and localized in distinct cells and tissues with specialized actin-based structures, such as growth cones of migrating neurons and stereocilia of inner ear hair cells. However, Caprice gene expression is varied among different cell types; especially enriched in several epithelial cells whereas relatively suppressed in a subset of epithelial cells, fibroblasts, and neuroblastoma cells at the transcriptional level. Thus, this protein is expected to be an effector for cell type-specific actin reorganization with its direct actin-binding properties and provides a novel model of cell morphology regulation by a non-ubiquitous single actin-bundling protein.
|
| 巻・号 |
19(4)
|
| ページ |
338-49
|
| 公開日 |
2014-4-1
|
| DOI |
10.1111/gtc.12131
|
| PMID |
24475924
|
| MeSH |
Actin Cytoskeleton / metabolism*
Actin Cytoskeleton / ultrastructure
Actins / metabolism*
Animals
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism*
Cells, Cultured
Dogs
Humans
Mice
Microfilament Proteins / genetics
Microfilament Proteins / metabolism*
Phosphoproteins / genetics
Phosphoproteins / metabolism*
Protein Binding
Protein Interaction Domains and Motifs
Pseudopodia / metabolism
|
| IF |
1.655
|
| 引用数 |
5
|
|
WOS 分野
|
GENETICS & HEREDITY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
A549
DU145(RCB2143)
MDCK(RCB0995)
NB1 |
