| RRC ID |
44882
|
| Author |
Kakazu N, Yamane H, Miyachi M, Shiwaku K, Hosoi H.
|
| Title |
Identification of the 12q15 amplicon within the homogeneously staining regions in the embryonal rhabdomyosarcoma cell line RMS-YM.
|
| Journal |
Cytogenet Genome Res
|
| Abstract |
Gene amplification represents one of the molecular mechanisms of oncogene overexpression in many types of tumors. Homogeneously staining regions (HSRs) are cytogenetic hallmarks of gene amplification. Rhabdomyosarcoma is the most common malignant soft-tissue tumor in children. RMS-YM is an embryonal rhabdomyosarcoma cell line that possesses 3 HSRs. This cytogenetic finding suggests the presence of gene amplifications associated with tumor development or progression in RMS-YM. Here, using fluorescence in situ hybridization, we detected high amplification of the MDM2 gene in the HSRs of RMS-YM. We also refined the region of the amplicon and identified that the FRS2 gene and others are amplified in RMS-YM. MDM2 and FRS2 play important roles as a regulator of p53 and a mediator of FGF signaling, respectively, and thus are potential molecular targets for therapy in many different tumors. RMS-YM may be useful for studies of the molecular pathways of tumorigenesis and tumor progression in rhabdomyosarcoma and for in vitro evaluation of newly developed therapeutic agents that target MDM2 or FRS2.
|
| Volume |
142(3)
|
| Pages |
167-73
|
| Published |
2014-1-1
|
| DOI |
10.1159/000357930
|
| PII |
000357930
|
| PMID |
24480864
|
| MeSH |
Adaptor Proteins, Signal Transducing / genetics
Cell Line, Tumor
Child, Preschool
Chromosome Aberrations
Chromosome Banding
Chromosomes, Human, Pair 12 / genetics*
Gene Amplification / genetics*
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Male
Membrane Proteins / genetics
Oncogenes*
Polymorphism, Single Nucleotide
Proto-Oncogene Proteins c-mdm2 / genetics*
Rhabdomyosarcoma, Embryonal / genetics*
Signal Transduction / genetics
|
| IF |
1.114
|
| Times Cited |
2
|
|
WOS Category
|
CELL BIOLOGY
GENETICS & HEREDITY
|
| Resource |
| Human and Animal Cells |
RMS-YM(RCB1695) |
