論文 - 詳細
RRC ID | 4490 |
---|---|
著者 | Hermansyah, Sugiyama M, Kaneko Y, Harashima S. |
タイトル | Yeast protein phosphatases Ptp2p and Msg5p are involved in G1-S transition, CLN2 transcription, and vacuole morphogenesis. |
ジャーナル | Arch Microbiol |
Abstract |
We previously reported that double disruption of protein phosphatase (PPase) genes PTP2 (phosphotyrosine-specific PPase) and MSG5 (phosphotyrosine and phosphothreonine/serine-PPase) causes Ca(2+) sensitive growth, whereas the single disruptions do not. This finding suggests that Ptp2p and Msg5p are involved in Ca(2+)-induced stress response in a redundant manner. To gain insight into the molecular mechanism causing calcium sensitivity of the ptp2 msg5 double disruptant, we performed fluorescence-activated cell sorting analysis and found a delayed G1 phase. This delayed G1 was consistent with the defect in bud emergence, and reduced CLN2 transcription upon addition of CaCl(2). We also found that Slt2p is hyper-phosphorylated in the Deltaptp2 Deltamsg5 double disruptant and that the vacuole of the Deltaptp2 Deltamsg5 double disruptant is fragmented even in the absence of Ca(2+). These findings suggest that both Ptp2p and Msg5p are involved in the G1 to S transition and vacuole morphogenesis possibly through their regulation of Slt2 pathway. |
巻・号 | 191(9) |
ページ | 721-33 |
公開日 | 2009-9-1 |
DOI | 10.1007/s00203-009-0498-3 |
PMID | 19680630 |
MeSH | Calcium Chloride / metabolism Cell Cycle Cyclins / biosynthesis* Gene Deletion Phosphoric Monoester Hydrolases / genetics Phosphoric Monoester Hydrolases / metabolism* Protein Tyrosine Phosphatases / genetics Protein Tyrosine Phosphatases / metabolism* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / growth & development Saccharomyces cerevisiae / physiology* Saccharomyces cerevisiae Proteins / biosynthesis* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Transcription, Genetic Vacuoles / physiology* |
IF | 1.884 |
引用数 | 11 |
WOS 分野 | MICROBIOLOGY |
リソース情報 | |
酵母 | BY5209 (= SH5209) BY5210 (= SH5210) |