| RRC ID |
44921
|
| 著者 |
Shen Y, Honma N, Kobayashi K, Jia LN, Hosono T, Shindo K, Ariga T, Seki T.
|
| タイトル |
Cinnamon extract enhances glucose uptake in 3T3-L1 adipocytes and C2C12 myocytes by inducing LKB1-AMP-activated protein kinase signaling.
|
| ジャーナル |
PLoS One
|
| Abstract |
We previously demonstrated that cinnamon extract (CE) ameliorates type 1 diabetes induced by streptozotocin in rats through the up-regulation of glucose transporter 4 (GLUT4) translocation in both muscle and adipose tissues. This present study was aimed at clarifying the detailed mechanism(s) with which CE increases the glucose uptake in vivo and in cell culture systems using 3T3-L1 adipocytes and C2C12 myotubes in vitro. Specific inhibitors of key enzymes in insulin signaling and AMP-activated protein kinase (AMPK) signaling pathways, as well as small interference RNA, were used to examine the role of these kinases in the CE-induced glucose uptake. The results showed that CE stimulated the phosphorylation of AMPK and acetyl-CoA carboxylase. An AMPK inhibitor and LKB1 siRNA blocked the CE-induced glucose uptake. We also found for the first time that insulin suppressed AMPK activation in the adipocyte. To investigate the effect of CE on type 2 diabetes in vivo, we further performed oral glucose tolerance tests and insulin tolerance tests in type 2 diabetes model rats administered with CE. The CE improved glucose tolerance in oral glucose tolerance tests, but not insulin sensitivity in insulin tolerance test. In summary, these results indicate that CE ameliorates type 2 diabetes by inducing GLUT4 translocation via the AMPK signaling pathway. We also found insulin antagonistically regulates the activation of AMPK.
|
| 巻・号 |
9(2)
|
| ページ |
e87894
|
| 公開日 |
2014-1-1
|
| DOI |
10.1371/journal.pone.0087894
|
| PII |
PONE-D-13-39530
|
| PMID |
24551069
|
| PMC |
PMC3925101
|
| MeSH |
3T3-L1 Cells
AMP-Activated Protein Kinases / metabolism*
Adipocytes / drug effects
Adipocytes / metabolism*
Animals
Body Weight / drug effects
Cinnamomum zeylanicum / chemistry*
Diabetes Mellitus, Type 2 / blood
Diabetes Mellitus, Type 2 / drug therapy
Diabetes Mellitus, Type 2 / metabolism
Diabetes Mellitus, Type 2 / pathology
Disease Models, Animal
Gene Knockdown Techniques
Glucose / metabolism*
Glucose Transporter Type 4 / metabolism
Insulin / blood
Insulin Resistance
Male
Mice
Muscle Cells / drug effects
Muscle Cells / metabolism*
Phosphorylation / drug effects
Plant Extracts / administration & dosage
Plant Extracts / pharmacology*
Plant Extracts / therapeutic use
Protein Kinase Inhibitors / pharmacology
Protein Serine-Threonine Kinases / metabolism*
Protein Transport / drug effects
Rats
Rats, Inbred OLETF
Signal Transduction / drug effects
|
| IF |
2.74
|
| 引用数 |
55
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
C2C12(RCB0987) |
