論文 - 詳細
| RRC ID | 44944 |
|---|---|
| 著者 | Odkhuu E, Koide N, Tsolmongyn B, Jambalganiin U, Naiki Y, Komatsu T, Yoshida T, Yokochi T. |
| タイトル | Involvement of redox balance in in vitro osteoclast formation of RAW 264.7 macrophage cells in response to LPS. |
| ジャーナル | Innate Immun |
| Abstract |
Here we report that LPS induces osteoclast (OC) formation in murine RAW 264.7 macrophage cells in RPMI-1640 medium but not in α-minimum essential medium (α-MEM) as the original culture medium. LPS-induced OC formation in both media was examined to clarify the differential response. Receptor activator of NF-κB ligand induced OC formation in either α-MEM or RPMI-1640 medium. However, LPS-induced OC formation in RAW 264.7 cells maintained in RPMI-1640 medium, but not α-MEM, which was also supported by mouse bone marrow-derived macrophages, although they were less sensitive to LPS than RAW 264.7 cells. LPS augmented the expression of nuclear factor of activated T-cells (NFATc1) as a key transcription factor of osteoclastogenesis in cells maintained in RPMI-1640 medium, but reduced it in cells maintained in α-MEM. A high concentration of LPS was cytotoxic against cells maintained in α-MEM. Glutathione exclusively present in RPMI-1640 medium prevented LPS-induced cell death in α-MEM and augmented LPS-induced NFATc1 expression, followed by enhanced LPS-induced OC formation. LPS induced higher generation of reactive oxygen species in α-MEM than RPMI-1640 medium. An antioxidant enhanced LPS-induced OC formation, whereas a pro-oxidant reduced it. Taken together, redox balance in the culture condition was suggested to regulate in vitro LPS-induced OC formation. |
| 巻・号 | 21(2) |
| ページ | 194-202 |
| 公開日 | 2015-2-1 |
| DOI | 10.1177/1753425914524242 |
| PII | 1753425914524242 |
| PMID | 24595208 |
| MeSH | Animals Cell Death Cell Differentiation Cell Line Culture Media / metabolism* Glutathione / metabolism* In Vitro Techniques Lipopolysaccharides / metabolism* Macrophages / physiology* Male Mice Mice, Inbred C57BL NF-kappa B / metabolism NFATC Transcription Factors / metabolism Osteoclasts / physiology* Oxidation-Reduction Reactive Oxygen Species / metabolism |
| IF | 2.298 |
| 引用数 | 6 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL BIOCHEMISTRY & MOLECULAR BIOLOGY MICROBIOLOGY IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |