RRC ID 45034
Author Shi M, Yang Y, Guan D, Zhang Y, Zhang Z.
Title Bioactivity of the crude polysaccharides from fermented soybean curd residue by Flammulina velutipes.
Journal Carbohydr Polym
Abstract The solid-state fermentation, reusing soybean curd residue (SCR) as a solid substrate, was conducted for producing polysaccharides by Flammulina velutipes (F. velutipes). The optimal fermentation conditions were 74.5% of moisture content, 9.69 of inoculum size and 30.27 of C/N ratio by response surface methodology. 59.15 mg/g of polysaccharides were obtained. F. velutipes polysaccharides were subsequently extracted from fermented SCR by ultrasonic assisted extraction. The optimal extract conditions were 30 min, 80 °C, 150 W and 20 of water to solid ratio and 106.74 mg/g of polysaccharides were obtained. Furthermore, the antioxidant and the immunomodulatory activities of polysaccharides were assessed. The results showed that polysaccharides exhibited a strong DPPH radical scavenging activity, SOD-like activity, stimulatory the proliferation of the macrophage, the production of nitric oxide, phagocytosis and the protection on Doxorubicin damage. These could lay the foundation for changing SCR into a nutritious functional food or a food additive.
Volume 89(4)
Pages 1268-76
Published 2012-8-1
DOI 10.1016/j.carbpol.2012.04.047
PII S0144-8617(12)00387-6
PMID 24750941
MeSH Animals Cell Line Cell Proliferation / drug effects Flammulina* / chemistry Flammulina* / metabolism Free Radical Scavengers* / chemistry Free Radical Scavengers* / isolation & purification Free Radical Scavengers* / metabolism Free Radical Scavengers* / pharmacology Fungal Polysaccharides* / biosynthesis Fungal Polysaccharides* / chemistry Fungal Polysaccharides* / isolation & purification Fungal Polysaccharides* / pharmacology Macrophages / cytology Macrophages / metabolism* Mice Nitric Oxide / biosynthesis Phagocytosis / drug effects Soy Foods / microbiology*
IF 6.044
Times Cited 22
WOS Category CHEMISTRY, APPLIED POLYMER SCIENCE CHEMISTRY, ORGANIC
Resource
Human and Animal Cells