Reference - Detail
|Author||Shi M, Yang Y, Guan D, Zhang Y, Zhang Z.|
|Title||Bioactivity of the crude polysaccharides from fermented soybean curd residue by Flammulina velutipes.|
The solid-state fermentation, reusing soybean curd residue (SCR) as a solid substrate, was conducted for producing polysaccharides by Flammulina velutipes (F. velutipes). The optimal fermentation conditions were 74.5% of moisture content, 9.69 of inoculum size and 30.27 of C/N ratio by response surface methodology. 59.15 mg/g of polysaccharides were obtained. F. velutipes polysaccharides were subsequently extracted from fermented SCR by ultrasonic assisted extraction. The optimal extract conditions were 30 min, 80 °C, 150 W and 20 of water to solid ratio and 106.74 mg/g of polysaccharides were obtained. Furthermore, the antioxidant and the immunomodulatory activities of polysaccharides were assessed. The results showed that polysaccharides exhibited a strong DPPH radical scavenging activity, SOD-like activity, stimulatory the proliferation of the macrophage, the production of nitric oxide, phagocytosis and the protection on Doxorubicin damage. These could lay the foundation for changing SCR into a nutritious functional food or a food additive.
|MeSH||Animals Cell Line Cell Proliferation / drug effects Flammulina* / chemistry Flammulina* / metabolism Free Radical Scavengers* / chemistry Free Radical Scavengers* / isolation & purification Free Radical Scavengers* / metabolism Free Radical Scavengers* / pharmacology Fungal Polysaccharides* / biosynthesis Fungal Polysaccharides* / chemistry Fungal Polysaccharides* / isolation & purification Fungal Polysaccharides* / pharmacology Macrophages / cytology Macrophages / metabolism* Mice Nitric Oxide / biosynthesis Phagocytosis / drug effects Soy Foods / microbiology*|
|WOS Category||CHEMISTRY, APPLIED POLYMER SCIENCE CHEMISTRY, ORGANIC|
|Human and Animal Cells|