| RRC ID |
45037
|
| Author |
Bamba Y, Shofuda T, Kanematsu D, Nonaka M, Yamasaki M, Okano H, Kanemura Y.
|
| Title |
Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Here we established a unique human glial cell line, GDC90, derived from a human glioma and demonstrated its utility as a glial scaffold for the polarization and differentiation of human induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs). When co-cultured with GDC90 cells, iPSC-NPCs underwent rapid polarization and neurite extension along the radially spreading processes of the GDC90 cells, and showed migratory behavior. This method is potentially useful for detailed examination of neurites or for controlling neurites behavior for regenerative medicine.
|
| Volume |
447(4)
|
| Pages |
683-8
|
| Published |
2014-5-16
|
| DOI |
10.1016/j.bbrc.2014.04.070
|
| PII |
S0006-291X(14)00712-8
|
| PMID |
24755070
|
| MeSH |
Cell Differentiation / physiology
Cell Line
Cell Line, Tumor
Cell Movement / physiology
Cell Polarity / physiology
Cell Shape
Coculture Techniques / methods
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / physiology*
Luminescent Proteins / genetics
Luminescent Proteins / metabolism
Membranes, Artificial
Nerve Regeneration
Neural Stem Cells / cytology*
Neural Stem Cells / physiology*
Neurites / physiology
Neuroglia / cytology*
Neuroglia / physiology*
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Regenerative Medicine / methods
Transfection
|
| IF |
2.985
|
| Times Cited |
8
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
|
